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Co-transfer of restriction endonucleases and plasmid DNA into mammalian cells by electroporation: effects on stable transformation.

作者信息

Yorifuji T, Mikawa H

机构信息

Department of Pediatrics, Faculty of Medicine, Kyoto University, Japan.

出版信息

Mutat Res. 1990 Feb;243(2):121-6. doi: 10.1016/0165-7992(90)90033-g.

DOI:10.1016/0165-7992(90)90033-g
PMID:2154689
Abstract

We co-transferred cloned HSV thymidine kinase gene and restriction endonucleases, HindIII, XbaI, or XhoI, into thymidine kinase-deficient mouse LtK- cells by electroporation. Stimulation of the transformation efficiency was observed with HindIII or XbaI, while little effect was observed with XhoI. The stimulation was observed for various forms of exogenous DNA (linear or circular plasmid DNA and single-stranded phage DNA) and correlated with the number of strand breaks in the host-cell DNA. These results suggest that the presence of DNA double-strand breaks stimulates the integration of exogenous DNA into host cell genomes.

摘要

相似文献

1
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Restriction enzymes increase efficiencies of illegitimate DNA integration but decrease homologous integration in mammalian cells.限制酶提高了哺乳动物细胞中非法DNA整合的效率，但降低了同源整合的效率。
Nucleic Acids Res. 2001 Dec 1;29(23):4826-33. doi: 10.1093/nar/29.23.4826.