Arambewela Lakshmi S R, Arawwawala L D A Menuka, Athauda Nandakumara
Industrial Technology Institute, Colombo, Sri Lanka.
J Ayurveda Integr Med. 2010 Jul;1(3):199-202. doi: 10.4103/0975-9476.72621.
Antioxidant and antifungal activity were determined for the essential oil of Alpinia calcarata Roscoe (Zingiberaceae) rhizomes. Its antioxidant properties were investigated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and thiobarbituric acid reactive substances (TBARS) assay. Butylated hydroxy toluene (BHT) and vitamin E served as positive controls. Antifungal activities were investigated against crop pathogens Curvularia spp. and Colletorichum spp. using the agar plate method. Fifty percent effective concentration (EC(50)) and % antioxidant index of the essential oil were 45 ± 0.4 and 16.1 ± 0.2 for DPPH and TBARS assays, respectively. The degree of, the essential oil's inhibition of the growth of crop pathogens Curvularia spp. and Colletorichum spp. varied with time period its effects were higher than greater than for the positive control, daconil. In conclusion, the essential oil of A. calcarata rhizomes possess moderate antioxidant property and promising antifungal activity.
测定了距瓣姜(姜科)根茎精油的抗氧化和抗真菌活性。通过2,2-二苯基-1-苦基肼(DPPH)自由基清除试验和硫代巴比妥酸反应性物质(TBARS)试验研究了其抗氧化性能。丁基羟基甲苯(BHT)和维生素E作为阳性对照。采用琼脂平板法研究了对作物病原菌弯孢属和炭疽菌属的抗真菌活性。在DPPH和TBARS试验中,精油的半数有效浓度(EC50)和抗氧化指数分别为45±0.4和16.1±0.2。精油对作物病原菌弯孢属和炭疽菌属生长的抑制程度随时间变化,其效果高于阳性对照百菌清。总之,距瓣姜根茎精油具有中等抗氧化性能和良好的抗真菌活性。