用于检测猪血清中猪流行性腹泻冠状病毒抗体的酶联免疫吸附测定。
Enzyme-linked immunosorbent assay for the detection of porcine epidemic diarrhea coronavirus antibodies in swine sera.
作者信息
Hofmann M, Wyler R
机构信息
Institute of Virology, University of Zurich, Switzerland.
出版信息
Vet Microbiol. 1990 Jan;21(3):263-73. doi: 10.1016/0378-1135(90)90037-v.
Abstract
An enzyme-linked immunosorbent assay (ELISA) for detecting serum antibodies to the porcine epidemic diarrhea coronavirus (PEDV) was established by using cell culture-grown PEDV as antigen for coating. Ultracentrifugation through 20 and 45% (w/w) sucrose cushions proved to be the best antigen purification method. Examination of 1024 swine sera showed a high specificity and a greater sensitivity of the ELISA, when compared with indirect immunofluorescence. Reference sera with high antibody titers to PEDV originated from two pigs experimentally infected with PEDV. Three different antigen purification methods and the advantages of the ELISA compared with an immunofluorescence test are discussed.
摘要
通过使用细胞培养增殖的猪流行性腹泻冠状病毒（PEDV）作为包被抗原，建立了一种用于检测血清中抗PEDV抗体的酶联免疫吸附测定（ELISA）。经20%和45%（w/w）蔗糖垫层超速离心被证明是最佳的抗原纯化方法。与间接免疫荧光法相比，对1024份猪血清的检测显示该ELISA具有高特异性和更高的灵敏度。高PEDV抗体效价的参考血清来源于两头经PEDV实验感染的猪。讨论了三种不同的抗原纯化方法以及该ELISA与免疫荧光试验相比的优点。
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Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea.用于检测患有猪流行性腹泻的猪体内类冠状病毒及其抗体的酶联免疫吸附测定。
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