用于检测患有猪流行性腹泻的猪体内类冠状病毒及其抗体的酶联免疫吸附测定。
Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea.
作者信息
Callebaut P, Debouck P, Pensaert M
出版信息
Vet Microbiol. 1982 Sep;7(4):295-306. doi: 10.1016/0378-1135(82)90009-8.
Abstract
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of the coronavirus-like agent in feces of pigs naturally affected with porcine epidemic diarrhea (PED) or experimentally infected with the CV777 isolate. The assay was specific and more sensitive than electron microscopy. An ELISA blocking assay is described for the detection and titration of antibodies. Specific antibody formation was demonstrated in pigs experimentally infected with CV777 and in swine naturally affected in PED.
摘要
开发了一种酶联免疫吸附测定（ELISA）法，用于检测自然感染猪流行性腹泻（PED）或经实验感染CV777毒株的猪粪便中的类冠状病毒病原体。该测定法具有特异性，且比电子显微镜检查更灵敏。还描述了一种用于检测和滴定抗体的ELISA阻断测定法。在经实验感染CV777的猪以及自然感染PED的猪中证实了特异性抗体的形成。
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