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Vet Microbiol. 1982 Sep;7(4):295-306. doi: 10.1016/0378-1135(82)90009-8.
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ELISA for the serology of FIP virus.猫传染性腹膜炎病毒血清学酶联免疫吸附试验。
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Comparison of direct electron microscopy, immune electron microscopy, and rotavirus enzyme-linked immunosorbent assay for detection of gastroenteritis viruses in children.用于检测儿童肠胃炎病毒的直接电子显微镜检查、免疫电子显微镜检查和轮状病毒酶联免疫吸附测定的比较
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Enzyme-linked immunosorbent assay for detection of antibody in volunteers experimentally infected with human coronavirus strain 229 E.用于检测实验感染人冠状病毒229E株志愿者体内抗体的酶联免疫吸附测定法。
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Experimental infection of pigs with a new porcine enteric coronavirus, CV 777.用一种新的猪肠道冠状病毒CV 777对猪进行实验性感染。
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An immunoelectron microscopic and immunofluorescent study on the antigenic relationship between the coronavirus-like agent, CV 777, and several coronaviruses.关于类冠状病毒CV 777与几种冠状病毒之间抗原关系的免疫电子显微镜和免疫荧光研究。
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Enzyme-linked immunosorbent assay for detection of antibodies to murine hepatitis virus.用于检测小鼠肝炎病毒抗体的酶联免疫吸附测定
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用于检测患有猪流行性腹泻的猪体内类冠状病毒及其抗体的酶联免疫吸附测定。

Enzyme-linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea.

作者信息

Callebaut P, Debouck P, Pensaert M

出版信息

Vet Microbiol. 1982 Sep;7(4):295-306. doi: 10.1016/0378-1135(82)90009-8.

DOI:10.1016/0378-1135(82)90009-8
PMID:7179716
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7117127/
Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of the coronavirus-like agent in feces of pigs naturally affected with porcine epidemic diarrhea (PED) or experimentally infected with the CV777 isolate. The assay was specific and more sensitive than electron microscopy. An ELISA blocking assay is described for the detection and titration of antibodies. Specific antibody formation was demonstrated in pigs experimentally infected with CV777 and in swine naturally affected in PED.

摘要

开发了一种酶联免疫吸附测定（ELISA）法，用于检测自然感染猪流行性腹泻（PED）或经实验感染CV777毒株的猪粪便中的类冠状病毒病原体。该测定法具有特异性，且比电子显微镜检查更灵敏。还描述了一种用于检测和滴定抗体的ELISA阻断测定法。在经实验感染CV777的猪以及自然感染PED的猪中证实了特异性抗体的形成。