Vassiliev Ivan, Vassilieva Svetlana, Truong Kam P, Beebe Luke F S, McIlfatrick Stephen M, Harrison Sharon J, Nottle Mark B
Reproductive Biotechnology Group, Robinson Institute, Centre for Stem Cell Research and School of Paediatrics and Reproductive Health, University of Adelaide, Australia.
Cell Reprogram. 2011 Jun;13(3):205-13. doi: 10.1089/cell.2010.0102. Epub 2011 May 7.
We report here the establishment and characterization of putative porcine embryonic stem cell (ESC) lines derived from somatic cell nuclear transfer embryos (NT-ESCs). These cells had a similar morphology to that described previously by us for ESCs derived from in vitro produced embryos, namely, a polygonal shape, a relatively small (10-15 μm) diameter, a small cytoplasmic/nuclear ratio, a single nucleus with multiple nucleoli and multiple lipid inclusions in the cytoplasm. NT-ESCs could be passaged at least 15 times and vitrified repeatedly without changes in their morphology, karyotype, or Oct-4 and Nanog expression. These cells formed embryoid bodies and could be directed to differentiate in vitro to cell types representative of all three germ layers. Following their injection into blastocysts, these cells preferentially localized in the inner cell mass. In conclusion, we have isolated putative porcine ESCs from cloned embryos that have the potential to be used for a variety of applications including as a model for human therapeutic cloning.
我们在此报告源自体细胞核移植胚胎（NT - ESCs）的假定猪胚胎干细胞（ESC）系的建立及特性。这些细胞的形态与我们之前描述的源自体外产生胚胎的胚胎干细胞相似，即呈多边形，直径相对较小（10 - 15μm），细胞质/细胞核比率小，单核且有多个核仁，细胞质中有多个脂质包涵体。NT - ESCs 至少可传代 15 次，并能反复进行玻璃化冷冻，而其形态、核型或 Oct - 4 和 Nanog 表达均无变化。这些细胞形成类胚体，并能在体外定向分化为代表所有三个胚层的细胞类型。将它们注射到囊胚后，这些细胞优先定位于内细胞团。总之，我们已从克隆胚胎中分离出假定的猪胚胎干细胞，其有潜力用于多种应用，包括作为人类治疗性克隆的模型。
