Department of Cardiac Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
Lipids Health Dis. 2011 May 8;10:68. doi: 10.1186/1476-511X-10-68.
Triglycerides is an independent risk factor for coronary artery disease (CAD) and is especially important in Indians because of high prevalence of hypertriglyceridemia in this population. Both genetic and environmental factors determine triglyceride levels. In a birth cohort from India, hypertriglyceridemia was found in 41% of men and 11% of women. Subjects who had high triglycerides had more rapid body mass index (BMI) or weight gain than rest of the cohort throughout infancy, childhood and adolescence. We analysed polymorphisms in APOA5, hepatic lipase and PPARγ genes and investigated their association with birth weight and serial changes in BMI.
Polymorphisms in APOA5 (-1131T > C, S19W), PPARγ (Pro12Ala) and hepatic lipase (-514C > T) were studied by polymerase chain reaction (PCR) followed by restriction digestion in 1492 subjects from the New Delhi Birth Cohort (NDBC). We assessed whether these polymorphisms influence lipid and other variables and serial changes in BMI, both individually and together.The risk allele of APOA5 (-1131C) resulted in 23.6 mg/dl higher triglycerides as compared to normal allele (P < 0.001). Risk allele of HL (-514T) was associated with significantly higher HDL2 levels (P = 0.002). Except for the marginal association of PPARγ Pro12Ala variation with a lower conditional weight at 6 months, (P = 0.020) and APOA5 S19W with a higher conditional BMI at 11 yrs of age (P = 0.030), none of the other associations between the gene polymorphisms and serial changes in body mass index from birth to young adulthood were significant.
The promoter polymorphism in APOA5 was associated with raised serum triglycerides and that of HL with raised HDL2 levels. None of the polymorphisms had any significant relationship with birth weight or serial changes in anthropometry from birth to adulthood in this cohort.
甘油三酯是冠心病（CAD）的一个独立危险因素，在印度人中尤为重要，因为该人群中高甘油三酯血症的患病率很高。遗传和环境因素共同决定甘油三酯水平。在印度的一个出生队列中，41%的男性和 11%的女性存在高甘油三酯血症。在整个婴儿期、儿童期和青春期，与队列中的其他成员相比，高甘油三酯血症患者的体重指数（BMI）或体重增加更快。我们分析了载脂蛋白 A5（APOA5）、肝脂肪酶和过氧化物酶体增殖物激活受体γ（PPARγ）基因的多态性，并研究了它们与出生体重和 BMI 序列变化的关系。
在来自新德里出生队列（NDBC）的 1492 名受试者中，通过聚合酶链反应（PCR）后限制性酶切分析研究了 APOA5（-1131T > C，S19W）、PPARγ（Pro12Ala）和肝脂肪酶（-514C > T）的多态性。我们评估了这些多态性是否会影响个体和整体的血脂和其他变量以及 BMI 的序列变化。APOA5 的风险等位基因（-1131C）导致甘油三酯比正常等位基因高 23.6mg/dl（P < 0.001）。HL 的风险等位基因（-514T）与更高的 HDL2 水平显著相关（P = 0.002）。除了 PPARγ Pro12Ala 变异与 6 个月时的条件体重略有相关（P = 0.020）以及 APOA5 S19W 与 11 岁时的条件 BMI 较高（P = 0.030）外，出生至成年期间体重指数的序列变化与基因多态性之间的其他关联均无统计学意义。
APOA5 的启动子多态性与血清甘油三酯升高有关，HL 的多态性与 HDL2 水平升高有关。在该队列中，没有任何多态性与出生体重或出生至成年期间的人体测量学序列变化有任何显著关系。
