Dendooven Amélie, Nguyen Tri Q, Brosens Lodewijk, Li Dongxia, Tarnow Lise, Parving Hans-Henrik, Rossing Peter, Goldschmeding Roel
Department of Pathology, University Medical Center Utrecht, Heidelberglaan 100, 3584CX Utrecht, The Netherlands.
J Negat Results Biomed. 2011 May 8;10:4. doi: 10.1186/1477-5751-10-4.
The -945GC polymorphism (rs6918698) in the connective tissue growth factor gene promoter (CTGF/CCN-2) has been associated with end organ damage in systemic sclerosis. Because CTGF is important in progression of diabetic kidney disease, we investigated whether the -945GC polymorphism is associated with plasma CTGF level and outcome in type 1 diabetes. The study cohort consisted of 448 diabetic nephropathy patients and 419 normoalbuminuric diabetic patients with complete data concerning renal function and cardiovascular characteristics. Genomic DNA was genotyped by a QPCR-based SNP assay. We observed no relation between the -945GC polymorphism and plasma CTGF level, and the genotype frequencies were not different in nephropathy patients vs. normoalbuminuric controls. General and cardiovascular mortality, and renal function decline was similar in patients with CC, CG or GG genotypes. In conclusion, the -945GC SNP does not affect plasma CTGF levels, incidence and prognosis of diabetic nephropathy, and cardiovascular outcome.
结缔组织生长因子基因启动子（CTGF/CCN - 2）中的 - 945G＞C多态性（rs6918698）与系统性硬化症的终末器官损伤有关。由于CTGF在糖尿病肾病进展中起重要作用，我们研究了 - 945G＞C多态性是否与1型糖尿病患者的血浆CTGF水平及预后相关。研究队列包括448例糖尿病肾病患者和419例尿白蛋白正常的糖尿病患者，他们有关于肾功能和心血管特征的完整数据。通过基于QPCR的SNP分析对基因组DNA进行基因分型。我们观察到 - 945G＞C多态性与血浆CTGF水平之间无关联，肾病患者与尿白蛋白正常对照组的基因型频率无差异。CC、CG或GG基因型患者的总体死亡率、心血管死亡率及肾功能下降情况相似。总之， - 945G＞C单核苷酸多态性不影响血浆CTGF水平、糖尿病肾病的发病率和预后以及心血管结局。
