Sutherland Christina A, Nicolau David P, Kuti Joseph L
Center for Anti-Infective Research and Development, Hartford Hospital, Hartford, CT 06102, USA.
J Chromatogr Sci. 2011 May;49(5):397-400. doi: 10.1093/chromsci/49.5.397.
An ultraviolet high-performance liquid chromatography (HPLC) method was developed to analyze anidulafungin in human plasma and saline. A reversed-phase column was used with a UV detector set at 310 nm. The mobile phase consisted of methanol and ammonium phosphate buffer at a flow rate of 1 mL/min. Micafungin was used as the internal standard. Both standard curves were linear over a range of 1 to 10 μg/mL. The intra-assay relative standard deviations (RSD) for plasma and saline matrices were 1.60-1.81% and 1.96-3.70%, respectively. The inter-assay RSD for plasma and saline matrices were 2.41-7.25% and 1.31-3.16%, respectively. This method successfully recapitulated anidulafungin plasma concentrations previously analyzed by HPLC-tandem mass spectrometry with precision and accuracy of 6.9% and 1.59%, respectively.
建立了一种紫外高效液相色谱（HPLC）法，用于分析人血浆和盐溶液中的阿尼芬净。使用反相柱，紫外检测器设置在310 nm。流动相由甲醇和磷酸铵缓冲液组成，流速为1 mL/min。米卡芬净用作内标。两条标准曲线在1至10μg/mL范围内均呈线性。血浆和盐溶液基质的批内相对标准偏差（RSD）分别为1.60 - 1.81%和1.96 - 3.70%。血浆和盐溶液基质的批间RSD分别为2.41 - 7.25%和1.31 - 3.16%。该方法成功重现了先前通过HPLC串联质谱分析的阿尼芬净血浆浓度，精密度和准确度分别为6.9%和1.59%。
