Clinical Pathology Laboratory Unit, Giannina Gaslini Institute, The Pediatric Hospital of Genoa, Via G. Gaslini 3-5, 16147, Genoa, Italy,
Anal Bioanal Chem. 2014 Feb;406(6):1795-8. doi: 10.1007/s00216-013-7590-x. Epub 2014 Jan 10.
Micafungin (MCF) is an antifungal agent of the echinocandin class approved in Europe both in adults and in children for the treatment of invasive candidiasis. Few analytical methods for therapeutic drug monitoring (TDM) of this drug have been described so far. In this paper, we describe a rapid and validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for the measurement of MCF in plasma. MCF was analyzed in 100-μL plasma samples over a wide range of concentrations (0.1-20 μg/mL) by LC-MS/MS after protein precipitation. The suitability of the assay for TDM was evaluated by using plasma samples from pediatric patients who received MCF for the treatment of invasive candidiasis. The overall turnaround time for the assay was 20 min. The lower limit of quantification of the method was 0.1 ng/mL. No ion suppression due to matrix effects was found with different pre-analytical conditions, such as hemolysis, lipemia, and hyperuricemia. A simple and rapid LC-MS/MS method which provides high specificity, precision, and accuracy for quantification of MCF in plasma has been developed and validated.
米卡芬净（MCF）是一种棘白菌素类抗真菌药物，在欧洲已被批准用于成人和儿童侵袭性念珠菌病的治疗。迄今为止，已经描述了几种用于该药物治疗药物监测（TDM）的分析方法。在本文中，我们描述了一种快速且经过验证的用于测定血浆中 MCF 的液相色谱-串联质谱（LC-MS/MS）分析方法。该方法通过 LC-MS/MS 在蛋白沉淀后分析了 100 μL 血浆样本中 MCF 的浓度范围（0.1-20 μg/mL）。使用接受 MCF 治疗侵袭性念珠菌病的儿科患者的血浆样本评估了该方法用于 TDM 的适用性。该分析方法的总分析时间为 20 分钟。该方法的定量下限为 0.1ng/mL。在不同的前处理条件下，如溶血、脂血和高尿酸血症，均未发现由于基质效应而导致的离子抑制。已经开发并验证了一种简单快速的 LC-MS/MS 方法，用于定量测定血浆中的 MCF，该方法具有很高的特异性、精密度和准确度。
