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Partial purification of L-ascorbate:ferricytochrome b5 oxidoreductase from rat liver microsomes.

作者信息

Scherer G, Weis W

出版信息

Hoppe Seylers Z Physiol Chem. 1977 Nov;358(11):1499-503. doi: 10.1515/bchm2.1977.358.2.1499.

Abstract

L-Ascorbate:ferricytochrome b5 oxidoreductase from rat liver microsomes was enriched about 750-fold after treatment with a non-ionic detergent with a yield of about 15%. The enriched enzyme still shows four bands in sodium dodecylsulfate polyacrylamide-gel electrophoresis and is a hydrophobic membrane protein.

摘要

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