Jeckel D, Karrenbauer A, Birk R, Schmidt R R, Wieland F
Institut für Biochemie I, Heidelberg, FRG.
FEBS Lett. 1990 Feb 12;261(1):155-7. doi: 10.1016/0014-5793(90)80659-7.
We have employed in vitro a truncated ceramide analogue with 8 carbon atoms in the sphingosine and the fatty acyl residue, each, to investigate the activity of various membrane fractions to synthesize truncated sphingomyelin. This shortened ceramide readily diffuses through membranes and therefore can easily find access to the lumina of intact organelles. Sphingomyelin synthase activity resides in the Golgi apparatus, and after sucrose density gradient centrifugation of Golgi-enriched fractions sphingomyelin synthesis follows a cis Golgi marker enzyme.
我们在体外使用了一种鞘氨醇和脂肪酰基残基中各含8个碳原子的截短神经酰胺类似物，以研究各种膜组分合成截短鞘磷脂的活性。这种缩短的神经酰胺很容易扩散通过膜，因此可以很容易地进入完整细胞器的内腔。鞘磷脂合酶活性存在于高尔基体中，在对富含高尔基体的组分进行蔗糖密度梯度离心后，鞘磷脂的合成遵循顺式高尔基体标记酶。
