Zentrum für Umweltforschung und nachhaltige Technologien, Fachbereich Biologie/Chemie, Abteilung Marine Mikrobiologie, Universität Bremen, Leobenerstraße, D-28359 Bremen, Germany.
Int J Syst Evol Microbiol. 2012 Mar;62(Pt 3):675-682. doi: 10.1099/ijs.0.030809-0. Epub 2011 May 6.
A Gram-staining-negative, aerobic to microaerophilic, rod-shaped, red-coloured bacterium, strain T4(T), was isolated from a freshwater pipe on Tenerife island. A polyphasic taxonomic study was performed in order to characterize the strain in detail. The isolate is surrounded by a slime capsule, occurs singly, in the form of short chains, or in aggregates, and exhibits catalase and oxidase activities. Growth was observed at 15-42 °C. Optimum growth occurred at pH 8 with mono- and disaccharides, followed by polysaccharides and deoxysaccharides, but the bacterium utilized only a restricted spectrum of alcohols, alditols, amides, amines, carboxylic acids and amino acids. Strain T4(T) tolerated concentrations of 0-4% (w/v) NaCl and contained MK-7 as predominant isoprenoid quinone as well as carotenoids, but lacked pigments of the flexirubin type. The predominant fatty acids were iso-C(15:0) (32.2%), summed feature 3 (C(16:1)ω6c and/or C(16:1)ω7c; 22.5%), and iso-C(17:0) 3-OH (7.9%). Major polar lipids were phosphatidylethanolamine, phospholipids, aminophospholipids and other lipids of unknown character. The DNA G+C content was approximately 41.8 mol%. The sequence of the 16S-rRNA gene assigned strain T4(T) to the CFB group, forming a coherent cluster with species of the genus Algoriphagus with the highest similarity of 98.8% to Algoriphagus aquatilis A8-7(T). DNA-DNA hybridization revealed 37.5% relatedness to strain A8-7(T). Based on morphological, physiological and molecular properties as well as on phylogenetic distinctiveness, strain T4(T) should be placed into the genus Algoriphagus as a novel species, for which the name Algoriphagus aquaeductus sp. nov. (type strain T4(T) =DSM 19759(T) =LMG 24398(T) =NCIMB 14399(T)) is proposed.
一株革兰氏阴性、需氧至微需氧、杆状、红色的细菌，菌株 T4(T)，从特内里费岛的一个淡水管道中分离得到。为了详细描述该菌株，进行了多相分类学研究。该分离株周围有一层黏液囊，以单个、短链或聚集的形式出现，并表现出过氧化氢酶和氧化酶活性。在 15-42°C 下观察到生长。最适生长在 pH 8 时，利用单糖和二糖，其次是多糖和脱氧糖，但细菌仅利用有限的醇、糖醇、酰胺、胺、羧酸和氨基酸谱。菌株 T4(T)耐受 0-4%（w/v）NaCl 的浓度，并含有 MK-7 作为主要的异戊烯醌以及类胡萝卜素，但缺乏弹性黄素型色素。主要脂肪酸为 iso-C(15:0)（32.2%）、总和特征 3（C(16:1)ω6c 和/或 C(16:1)ω7c；22.5%）和 iso-C(17:0) 3-OH（7.9%）。主要极性脂为磷脂酰乙醇胺、磷脂、氨基磷脂和其他未知性质的脂类。DNA G+C 含量约为 41.8 mol%。16S-rRNA 基因序列将菌株 T4(T)归入 CFB 组，与 Algoriphagus 属的物种形成一个具有最高相似度为 98.8%的 Algoriphagus aquatilis A8-7(T)的连贯聚类。DNA-DNA 杂交显示与菌株 A8-7(T)的相关性为 37.5%。基于形态、生理和分子特性以及系统发育的独特性，菌株 T4(T)应归入 Algoriphagus 属，作为一个新物种，命名为 Algoriphagus aquaeductus sp. nov.（模式菌株 T4(T) =DSM 19759(T) =LMG 24398(T) =NCIMB 14399(T)）。
