Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA 94720, USA.
Department of Earth, Atmospheric, and Planetary Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Int J Syst Evol Microbiol. 2013 Jan;63(Pt 1):163-168. doi: 10.1099/ijs.0.038646-0. Epub 2012 Feb 24.
A Gram-negative, non-motile, non-spore-forming bacterial strain, PR1(T), was isolated from a mud core sample containing colonial choanoflagellates near Hog Island, Virginia, USA. Strain PR1(T) grew optimally at 30 °C and with 3 % (w/v) NaCl. Strain PR1(T) contained MK-7 as the major menaquinone as well as carotenoids but lacked pigments of the flexirubin-type. The predominant fatty acids were iso-C(15 : 0) (29.4 %), iso-C(17 : 1)ω9c (18.5 %) and summed feature 3 (C(16 : 1)ω6c and/or C(16 : 1)ω7c; 11.3 %). The major polar lipids detected in strain PR1(T) were phosphatidylethanolamine, an unknown phospholipid, an aminophospholipid, an aminolipid and two lipids of unknown character. The DNA G+C content was 38.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain PR1(T) fell within the cluster comprising the genus Algoriphagus and was most closely related to Algoriphagus halophilus JC 2051(T) (95.4 % sequence similarity) and Algoriphagus lutimaris S1-3(T) (95.3 % sequence similarity). The 16S rRNA gene sequence similarity between strain PR1(T) and the type strains of other species of the genus Algoriphagus were in the range 91-95 %. Differential phenotypic properties and phylogenetic and genetic distinctiveness of strain PR1(T) demonstrated that this strain was distinct from other members of the genus Algoriphagus, including its closest relative, A. halophilus. Based on phenotypic, chemotaxonomic, phylogenetic and genomic data, strain PR1(T) should be placed in the genus Algoriphagus as a representative of a novel species, for which the name Algoriphagus machipongonensis sp. nov. is proposed. The type strain is PR1(T) (= ATCC BAA-2233(T) = DSM 24695(T)).
一株革兰氏阴性、非运动、非孢子形成的细菌菌株 PR1(T)，从美国弗吉尼亚州霍格岛附近含有殖民地领鞭毛藻的泥芯样本中分离得到。菌株 PR1(T) 在 30°C 和 3%（w/v）NaCl 的条件下生长最佳。菌株 PR1(T) 含有 MK-7 作为主要的甲萘醌，还含有类胡萝卜素，但缺乏 flexirubin 型色素。主要脂肪酸为 iso-C(15 : 0)（29.4%）、iso-C(17 : 1)ω9c（18.5%）和总和特征 3（C(16 : 1)ω6c 和/或 C(16 : 1)ω7c；11.3%）。在菌株 PR1(T) 中检测到的主要极性脂为磷脂酰乙醇胺、一种未知的磷脂、一种氨基磷脂、一种氨基脂和两种性质未知的脂。DNA G+C 含量为 38.7 mol%。基于 16S rRNA 基因序列的系统发育分析表明，菌株 PR1(T) 属于 Algoriphagus 属的聚类，与 Algoriphagus halophilus JC 2051(T)（95.4%序列相似性）和 Algoriphagus lutimaris S1-3(T)（95.3%序列相似性）最为密切相关。菌株 PR1(T) 与 Algoriphagus 属其他种的模式菌株的 16S rRNA 基因序列相似性在 91-95%之间。菌株 PR1(T) 的表型特性、化学分类特性、系统发育和遗传特征表明，该菌株与 Algoriphagus 属的其他成员不同，包括其最接近的亲缘关系 A. halophilus。基于表型、化学分类、系统发育和基因组数据，菌株 PR1(T) 应被归入 Algoriphagus 属，作为一个新种的代表，提议将其命名为 Algoriphagus machipongonensis sp. nov.。模式菌株为 PR1(T)（=ATCC BAA-2233(T)=DSM 24695(T)）。
