China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Int J Syst Evol Microbiol. 2012 Jul;62(Pt 7):1630-1635. doi: 10.1099/ijs.0.033902-0. Epub 2011 Sep 2.
A novel pale-pink-coloured strain, designated R9-65(T), was isolated from a tundra soil near Ny-Ålesund, Svalbard Archipelago, Norway (78° N). The cells were facultatively anaerobic, Gram-staining-negative, non-motile and rod-shaped. Growth occurred at 4-32 °C (optimum, 25-28 °C), at pH 5.0-9.0 (optimum, pH 6.0-7.0) and with 0-1.0% (w/v) NaCl (optimum, no NaCl). Flexirubin-type pigments were absent. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R9-65(T) belonged to the genus Mucilaginibacter in the family Sphingobacteriaceae. The 16S rRNA gene sequence similarity between strain R9-65(T) and type strains of related species ranged from 93.4 to 96.6%. Strain R9-65(T) contained summed feature 3 (C(16:1)ω7c and/or C(16:1)ω6c, 34.3%) and iso-C(15:0) (20.3%) as major cellular fatty acids, MK-7 as the major respiratory quinone, and phosphatidylethanolamine as the main polar lipid. The DNA G+C content of strain R9-65(T) was 47.2 mol%. On the basis of phylogenetic, physiological and chemotaxonomic data, strain R9-65(T) is considered to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter soli sp. nov. is proposed. The type strain is R9-65(T) (=CCTCC AB 2010331(T)=NRRL B-59458(T)).
一株新型浅粉色菌株，命名为 R9-65(T)，分离自挪威斯瓦尔巴群岛新奥尔松附近的苔原土壤（78°N）。细胞为兼性厌氧菌，革兰氏染色阴性，无动力，呈杆状。生长温度为 4-32°C（最适温度为 25-28°C），pH 值为 5.0-9.0（最适 pH 值为 6.0-7.0），盐度为 0-1.0%（w/v）（最适盐度为无盐）。不存在 flexirubin 型色素。基于 16S rRNA 基因序列的系统发育分析表明，菌株 R9-65(T)属于鞘脂单胞菌科粘质沙雷氏菌属。菌株 R9-65(T)与相关种的模式菌株的 16S rRNA 基因序列相似性为 93.4%至 96.6%。菌株 R9-65(T)含有 summed feature 3（C(16:1)ω7c 和/或 C(16:1)ω6c，34.3%）和 iso-C(15:0)（20.3%）作为主要细胞脂肪酸，MK-7 作为主要呼吸醌，磷脂酰乙醇胺作为主要极性脂。菌株 R9-65(T)的 DNA G+C 含量为 47.2mol%。基于系统发育、生理和化学分类学数据，菌株 R9-65(T)被认为代表粘质沙雷氏菌属的一个新种，因此提议将其命名为土壤粘质沙雷氏菌。模式菌株为 R9-65(T)=CCTCC AB 2010331(T)=NRRL B-59458(T)。
