Institution for Applied and Environmental Microbiology of Life Sciences College, Inner Mongolia Agricultural University, Hohhot 010018, PR China.
Key Laboratory of Dairy Biotechnology and Engineer, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, 010018, PR China.
Int J Syst Evol Microbiol. 2012 Mar;62(Pt 3):647-653. doi: 10.1099/ijs.0.031286-0. Epub 2011 May 6.
Two bacterial isolates from the surface water of a haloalkaline lake on the Mongolia Plateau, strains MIM18(T) and MIM19, were characterized; their morphological, physiological and chemotaxonomic characters, and phylogenetic position based on 16S rRNA gene sequences, were determined. The two strains were alkaliphilic, halotolerant, non-motile, aerobic, Gram-negative, orange-red, rod-shaped and oxidase-positive. Growth was observed in 0-5.5% (w/v) NaCl, with optimum growth at 1%. The temperature range for growth was 0-41 °C, with good growth at 28-37 °C and optimum growth at 30-33 °C. The DNA G+C content was 39.8-41.2 mol%. The strains contained menaquinone MK-7 as the major respiratory quinone and phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine as the major polar lipids. Predominant cellular fatty acids were iso-C(15:0) (28.1-29.3%), iso-C(17:0) 3-OH (14.6-18.8%), C(15:1)ω6c (5.3-8.6%), C(14:0) 2-OH and/or iso-C(15:0) 2-OH (5.4-6.1%), and iso-C(17:1)ω9c and/or C(16:0) 10-methyl (5.0-6.8%). 16S rRNA gene sequence analysis showed that Belliella and Nitritalea of the family Cyclobacteriaceae were the closest related species with sequence similarities of 91.7-92.3% and 88.2%, respectively, with strains of these genera; other members of the Cyclobacteriaceae had sequence similarities lower than 88%. Phylogenetic analysis indicated that the strains formed a deep-rooted lineage distinct from the clades represented by the genera Belliella, Nitritalea, Indibacter, Aquiflexum, Echinicola, Litoribacter, Cyclobacterium and Algoriphagus. Based on the phenotypic and phylogenetic characteristics mentioned above, the two strains are representatives of a single novel species in a new genus; the name Mongoliitalea lutea gen. nov., sp. nov. is proposed, with MIM18(T) (=ACCC 05421(T)=KCTC 23224(T)) as the type strain.
从蒙古高原的一个盐湖的地表水分离得到 2 株细菌，菌株 MIM18(T) 和 MIM19，对其进行了特征描述；基于 16S rRNA 基因序列，测定了它们的形态学、生理学和化学分类特征以及系统发育地位。这两株菌都是嗜碱、耐盐、非运动、需氧、革兰氏阴性、橙红色、杆状、氧化酶阳性。在 0-5.5%（w/v）NaCl 中生长，最适生长在 1%。生长温度范围为 0-41°C，在 28-37°C 下生长良好，最适生长在 30-33°C。DNA G+C 含量为 39.8-41.2mol%。菌株含有menaquinone MK-7 作为主要呼吸醌和磷脂酰胆碱、磷脂酰甘油和磷脂酰乙醇胺作为主要极性脂。主要的细胞脂肪酸是 iso-C(15:0)（28.1-29.3%）、iso-C(17:0) 3-OH（14.6-18.8%）、C(15:1)ω6c（5.3-8.6%）、C(14:0) 2-OH 和/或 iso-C(15:0) 2-OH（5.4-6.1%）和 iso-C(17:1)ω9c 和/或 C(16:0) 10-methyl（5.0-6.8%）。16S rRNA 基因序列分析表明，与该属最接近的种是Cyclobacteriaceae 科的Belliella 和 Nitritalea，序列相似性分别为 91.7-92.3%和 88.2%，与这些属的菌株；该科的其他成员的序列相似性低于 88%。系统发育分析表明，这些菌株形成了一个与代表属 Belliella、Nitritalea、Indibacter、Aquiflexum、Echinicola、Litoribacter、Cyclobacterium 和 Algoriphagus 的分支明显不同的深根系谱系。基于上述表型和系统发育特征，这两株菌代表了一个新属中的一个新种；提议建立一个新属，名为 Mongoliitalea lutea gen. nov.，sp. nov.，以 MIM18(T)=ACCC 05421(T)=KCTC 23224(T)为模式菌株。
