Division of Applied Marine Biotechnology and Engineering, Faculty of Marine Bioscience and Technology, Gangneung-Wonju National University, Gangneung, 210-702, Republic of Korea.
Korea Institute of Science and Technology, Gangneung, Gangwondo, 210-340, Republic of Korea.
Int J Syst Evol Microbiol. 2012 Mar;62(Pt 3):624-631. doi: 10.1099/ijs.0.028738-0. Epub 2011 May 6.
A Gram-negative, aerobic, ovoid to rod-shaped bacterial strain, KME 002(T) was isolated from a marine ascidian, Halocynthia roretzi, off the coast of Gangneung, Korea. Phylogenetic analyses based on 16S rRNA gene sequences showed that this strain belonged to the family Rhodobacteraceae in the class Alphaproteobacteria and was closely related to the type strains of Dinoroseobacter shibae, Roseovarius crassostreae and Pseudoruegeria aquimaris with 95.0, 94.7 and 94.5% 16S rRNA gene sequence similarities, respectively. KME 002(T) was an obligately halophilic bacterium requiring 1 to 5% (w/v) NaCl, with an absolute requirement for magnesium chloride for growth. Cells were motile by means of a single polar flagellum and showed budding fission. The predominant cellular fatty acid of the isolate was C(18:1)ω7c and Q-10 was the major ubiquinone. The DNA G+C content of the strain was 71.6 mol%. The major secondary metabolites from cultures in liquid medium were cholic acid derivatives, including 3α,12α-hydroxy-3-keto-glycocholanic acid, 12-hydroxy-3-keto-glycocholanic acid, nutriacholic acid and deoxycholic acid. These characteristics determined in this polyphasic study suggest that strain KME 002(T) represents a novel species in a new genus of the family Rhodobacteraceae. The name Hasllibacter halocynthiae gen. nov., sp. nov. is proposed for this isolate, and the type strain is KME 002(T) (=JCM 16214(T)=KCCM 90082(T)).
一株革兰氏阴性、需氧、卵形到杆状的细菌菌株 KME 002(T) 是从韩国江陵沿海的一种海洋海鞘 Halocynthia roretzi 中分离出来的。基于 16S rRNA 基因序列的系统发育分析表明，该菌株属于α变形菌纲红杆菌科，与 Dinoroseobacter shibae、Roseovarius crassostreae 和 Pseudoruegeria aquimaris 的模式菌株最为密切，其 16S rRNA 基因序列相似性分别为 95.0%、94.7%和 94.5%。KME 002(T) 是一种严格的嗜盐菌，需要 1%至 5%（w/v）的 NaCl，生长绝对需要氯化镁。细胞通过单一极鞭毛运动，并表现出出芽分裂。该分离株的主要细胞脂肪酸为 C(18:1)ω7c，主要的醌类为 Q-10。该菌株的 DNA G+C 含量为 71.6 mol%。在液体培养基中培养的主要次生代谢产物是胆酸衍生物，包括 3α,12α-羟基-3-酮-甘胆酸、12-羟基-3-酮-甘胆酸、营养胆酸和脱氧胆酸。这些在多相研究中确定的特征表明，菌株 KME 002(T) 代表红杆菌科一个新属的一个新种。提议将该分离株命名为 Hasllibacter halocynthiae gen. nov.，sp. nov.，其模式菌株为 KME 002(T)=JCM 16214(T)=KCCM 90082(T)。
