从海水中分离得到的新属新种浮游玫瑰异养菌，以及将玫瑰异养菌属的三个物种重新分类为厚壳贻贝玫瑰异养菌新组合、太平洋海鞘玫瑰异养菌新组合和潮间带沉积物玫瑰异养菌新组合。
Aliiroseovarius pelagivivens gen. nov., sp. nov., isolated from seawater, and reclassification of three species of the genus Roseovarius as Aliiroseovarius crassostreae comb. nov., Aliiroseovarius halocynthiae comb. nov. and Aliiroseovarius sediminilitoris comb. nov.
作者信息
Park Sooyeon, Park Ji-Min, Kang Chul-Hyung, Yoon Jung-Hoon
机构信息
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
出版信息
Int J Syst Evol Microbiol. 2015 Aug;65(8):2646-2652. doi: 10.1099/ijs.0.000315. Epub 2015 May 11.
A Gram-stain-negative, non-motile, aerobic and ovoid or rod-shaped bacterium, designated GYSW-22T, was isolated from seawater off Geoje Island in the South Sea, South Korea. Strain GYSW-22T grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 1.0-2.0% (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain GYSW-22T and the type strains of Roseovarius crassostreae, Roseovarius halocynthiae and Roseovarius sediminilitoris form a coherent cluster, independent of phylogenetic lineages or clusters comprising the type strains of other species of the genus Roseovarius. Strain GYSW-22T exhibited 16S rRNA gene sequence similarities of 97.2, 96.6 and 96.3% to R. halocynthiae MA1-10T, R. crassostreae CV919-312T and R. sediminilitoris M-M10T, respectively, and of 92.6-94.7% to the type strains of other species of the genus Roseovarius. Strain GYSW-22T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain GYSW-22T was 59.0 mol% and its mean DNA-DNA relatedness value with R. halocynthiae MA1-10T was 15 %. On the basis of the data presented, we propose strain GYSW-22T represents a novel species of a new genus, Aliiroseovarius pelagivivens gen. nov., sp. nov. The type strain of the type species is GYSW-22T ( = KCTC 42459T = CECT 8811T). In this study, it is also proposed that Roseovarius crassostreae, Roseovarius halocynthiae and Roseovarius sediminilitoris be reclassified into the new genus as Aliiroseovarius crassostreae comb. nov. (type strain CV919-312T = ATCC BAA-1102T = DSM 16950T), Aliiroseovarius halocynthiae comb. nov. (type strain MA1-10T = KCTC 23462T = CCUG 60745T) and Aliiroseovarius sediminilitoris comb. nov. (type strain M-M10T = KCTC 23959T = CCUG 62413T), respectively.
从韩国南海巨济岛附近海域分离出一株革兰氏染色阴性、无运动性、需氧的卵形或杆状细菌，命名为GYSW-22T。菌株GYSW-22T在25 °C、pH 7.0 - 8.0以及1.0 - 2.0%（w/v）NaCl条件下生长最佳。基于16S rRNA基因序列构建的邻接法、最大似然法和最大简约法系统发育树表明，菌株GYSW-22T与厚壳贻贝玫瑰弧菌（Roseovarius crassostreae）、盐渍玫瑰弧菌（Roseovarius halocynthiae）和沉积物玫瑰弧菌（Roseovarius sediminilitoris）的模式菌株形成一个连贯的簇，独立于玫瑰弧菌属其他物种模式菌株所在的系统发育谱系或簇。菌株GYSW-22T与盐渍玫瑰弧菌MA1-10T、厚壳贻贝玫瑰弧菌CV919-312T和沉积物玫瑰弧菌M-M10T的16S rRNA基因序列相似性分别为97.2%、96.6%和96.3%，与玫瑰弧菌属其他物种模式菌株的相似性为92.6 - 94.7%。菌株GYSW-22T以Q-10作为主要泛醌，以C18 : 1ω7c作为主要脂肪酸。主要极性脂为磷脂酰胆碱、磷脂酰甘油、一种未鉴定的氨基脂和一种未鉴定的脂。菌株GYSW-22T的DNA G+C含量为59.0 mol%，其与盐渍玫瑰弧菌MA1-10T的平均DNA-DNA杂交值为15 %。基于所提供的数据，我们提议菌株GYSW-22T代表一个新属的新物种，定名为海洋栖居玫瑰异弧菌（Aliiroseovarius pelagivivens），属名新拟，种名新拟。模式种的模式菌株为GYSW-22T（ = KCTC 42459T = CECT 8811T）。在本研究中，还提议将厚壳贻贝玫瑰弧菌、盐渍玫瑰弧菌和沉积物玫瑰弧菌重新分类到新属中，分别为厚壳贻贝海洋栖居玫瑰异弧菌（Aliiroseovarius crassostreae），组合新拟（模式菌株CV919-312T = ATCC BAA-1102T = DSM 16950T）、盐渍海洋栖居玫瑰异弧菌（Aliiroseovarius halocynthiae），组合新拟（模式菌株MA1-10T = KCTC 23462T = CCUG 60745T）和沉积物海洋栖居玫瑰异弧菌（Aliiroseovarius sediminilitoris），组合新拟（模式菌株M-M10T = KCTC 23959T = CCUG 62413T）。
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