Center for Biomedical Engineering and Department of Anatomy and Neurobiology, University of Kentucky, Lexington, KY 40506-0070 (U.S.A.).
Restor Neurol Neurosci. 1990 Jan 1;1(3):303-9. doi: 10.3233/RNN-1990-13419.
The influence of non-invasive, low level, pulsed electromagnetic fields (PEMF) on regeneration was tested on in vitro and in vivo models. Cultures of dorsal root ganglia were exposed to 2 Hz PEMF, amplitude of 0.05 mTesla while rats after a 'crush' lesion of sciatic nerves were exposed to 2 Hz PEMF, amplitude of 0.3 mTesla. In in vitro experiments, relative to controls, cultures treated with PEMF exhibited a significant increase in neurite outgrowth with dense labeling of neurons and neurites on autoradiographs after incorporation of [3H]proline into new proteins. In vivo exposure of rats to PEMF for 3, 4 or 6 days after lesioning produced a 22% increase in the regeneration rate relative to controls with no effect on the initial delay period. When rats were exposed to PEMF before lesioning without further treatment, the same degree of stimulation of axonal sprouting was obtained. Reduction of the amplitude from 0.3 mTesla to 0.06 mTesla eliminated this pre-exposure response. Alterations in the distribution of new proteins synthesized 2 weeks after PEMF treatment provide additional evidence for its influence at the whole body and cellular levels.
非侵入性、低水平、脉冲电磁场（PEMF）对再生的影响已在体外和体内模型中进行了测试。背根神经节培养物暴露于 2 Hz PEMF，幅度为 0.05 mTesla，而坐骨神经“挤压”损伤后的大鼠则暴露于 2 Hz PEMF，幅度为 0.3 mTesla。在体外实验中，与对照组相比，用 PEMF 处理的培养物表现出神经突生长的显著增加，并且在将 [3H]脯氨酸掺入新蛋白质后，在放射自显影上神经元和神经突的密集标记。在损伤后 3、4 或 6 天对大鼠进行体内 PEMF 暴露会使再生率相对于对照组增加 22%，而对初始延迟期没有影响。当大鼠在损伤前暴露于 PEMF 而没有进一步治疗时，也获得了相同程度的轴突发芽刺激。将幅度从 0.3 mTesla 降低到 0.06 mTesla 消除了这种预暴露反应。在 PEMF 处理后 2 周合成的新蛋白质的分布变化为其在全身和细胞水平的影响提供了额外的证据。
