Division of Orthopaedic Surgery, Center for BiomedicaL Engineering, University of Kentucky, Shriners Hospitals for Crippled Children, 1900 Richmond Road, Lexington, KY, USA.
Restor Neurol Neurosci. 1994 Jan 1;6(3):189-93. doi: 10.3233/RNN-1994-6303.
Footprint analysis is a non-invasive method to quantitate functional recovery after crush injury in the rat sciatic nerve model. Traditional methods of producing the footprints for measurement are limited by inability to reliably produce clear prints when the injury is severe. We describe the use of video technique with image analysis to record and measure these prints. Video had fewer unmeasurable prints than ink. For the 1-5 and 2-4 toe spreads, there was good correlation of video measurements with ink method and better repeatability using video as compared with ink. However, the print length parameter determined by video had poorer repeatability and poorly correlated with that measured by ink. Therefore, calculation of a Sciatic Function Index by video is not appropriate. Since the print length also varies with gait velocity, we believe that a ratio of injured:uninjured hindfoot 1-5 toe spreads as measured by video is a more reliable and repeatable measure of functional recovery in this model.
足迹分析是一种非侵入性的方法，可定量评估大鼠坐骨神经模型挤压伤后的功能恢复。传统的足迹制作方法在损伤严重时无法可靠地产生清晰的印迹。我们描述了使用视频技术和图像分析来记录和测量这些印迹。与墨迹法相比，视频法的不可测量印迹更少。对于 1-5 和 2-4 趾间距，视频测量与墨迹法具有良好的相关性，并且与墨迹法相比，视频法具有更好的可重复性。然而，视频法确定的印迹长度参数的可重复性较差，与墨迹法测量的相关性也较差。因此，通过视频计算坐骨功能指数是不合适的。由于印迹长度也随步态速度而变化，我们认为，通过视频测量的受伤：未受伤后足 1-5 趾间距的比值是该模型中功能恢复的更可靠和可重复的测量方法。
