Unidad de Neurobiología Molecular, Universidad Católica, Casilla 114-D, Santiago (Chile).
Restor Neurol Neurosci. 1993 Jan 1;5(3):181-9. doi: 10.3233/RNN-1993-5302.
In rat sciatic nerves, IGF-I or bFGF was applied distal to a crush to evaluate their effects upon the restoration of the neuromuscular function. In comparison with the recovery following a simple crush, treatment with growth factors resulted in (i) enhanced elongation of regenerating axons ( + 24%) up to day 3 post lesion (PL); (ii) more sprouts at early times; (iii) reduced participation of macrophages in the removal of the degenerating myelin in the first week PL; (iv) restoration of the neuromuscular transmission 2 days earlier; (v) a prolonged relaxation time and a reduced specific tetanic tension at week 3 PL but not at week 7 PL. Other indicators of recovery such as conduction velocity of nerve impulse, muscle weight, specific twitch tension, and time to peak were not affected by bFGF or IGF-I. Results suggest that IGF-I and bFGF affect locally Schwann cells and axons, and also the neuron as a whole, including its trophic function. We conclude that IGF-I and bFGF applied to the nerve, albeit moderately, improve the recovery of the neuromuscular function.
在大鼠坐骨神经中，IGF-I 或 bFGF 被施加到压碎部位的远端，以评估它们对神经肌肉功能恢复的影响。与单纯压碎后的恢复相比，生长因子的治疗导致：（i）再生轴突的伸长增加（+24%），直至损伤后第 3 天（PL）；（ii）早期有更多的芽生；（iii）在 PL 的第一周内，巨噬细胞参与清除脱髓鞘的程度降低；（iv）神经肌肉传递恢复提前 2 天；（v）PL 第 3 周时松弛时间延长，特定强直张力降低，但 PL 第 7 周时没有变化。神经冲动传导速度、肌肉重量、特定抽搐张力和峰值时间等其他恢复指标不受 bFGF 或 IGF-I 的影响。结果表明，IGF-I 和 bFGF 局部影响施万细胞和轴突，也影响神经元整体，包括其营养功能。我们得出结论，IGF-I 和 bFGF 应用于神经，尽管是适度的，也能改善神经肌肉功能的恢复。
