通过整合 pSC101 衍生的接合质粒构建的新型大肠杆菌 Hfr 菌株的特性。
Properties of new Escherichia coli Hfr strains constructed by integration of pSC101-derived conjugative plasmids.
作者信息
François V, Conter A, Louarn J M
机构信息
Centre de Biochimie et de Génétique cellulaires du Centre National de la Recherche Scientifique, Toulouse, France.
出版信息
J Bacteriol. 1990 Mar;172(3):1436-40. doi: 10.1128/jb.172.3.1436-1440.1990.
Abstract
Conjugative temperature-sensitive plasmids were derived from pSC101. These plasmids are useful in genetic analysis for two reasons: (i) they render possible the construction of new Hfr lines by plasmid integration at predetermined chromosomal loci via Tn10 inverse transposition, and (ii) the Hfr characters are transducible via bacteriophage P1. We also showed that replication from pSC101 origin is deleterious for the plasmid-chromosome fusion.
摘要
接合型温度敏感质粒源自pSC101。这些质粒在遗传分析中很有用，原因有两个：（i）它们使得通过Tn10反向转座在预定染色体位点整合质粒来构建新的高频重组（Hfr）品系成为可能；（ii）Hfr特性可通过噬菌体P1进行转导。我们还表明，从pSC101原点进行复制对质粒-染色体融合是有害的。
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2
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J Bacteriol. 1983 Oct;156(1):205-11. doi: 10.1128/jb.156.1.205-211.1983.
3
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J Mol Biol. 1984 Mar 15;173(4):437-61. doi: 10.1016/0022-2836(84)90390-5.
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