Institute of Biomedicine, Physiology, University of Eastern Finland, Kuopio, Finland.
Med Sci Sports Exerc. 2011 Dec;43(12):2368-74. doi: 10.1249/MSS.0b013e3182220179.
We clarified the effect of insulin-like growth factor-1 (IGF1), IGF-binding protein-3 (IGFBP3), interleukin-6 (IL6), and its receptor (IL6R) gene variants on muscular and aerobic performance, body composition, and on circulating levels of IGF-1 and IL-6. Single nucleotide polymorphisms (SNPs) may, in general, influence gene regulation or its expression, or the structure and function of the corresponding protein, and modify its biological effects. IGF-1 is involved in the anabolic pathways of skeletal muscle. IL-6 plays an important role in muscle energy homeostasis during strenuous physical exercise.
Eight hundred forty-one healthy Finnish male subjects of Caucasian origin were genotyped for IGF1 (rs6220 and rs7136446), IGFBP3 (rs2854744), IL6 (rs1800795), and IL6R (rs4537545) SNPs, and studied for associations with maximal force of leg extensor muscles, maximal oxygen consumption, body fat percent, and IGF-1 and IL-6 levels. Analytic methods included dynamometer, bicycle ergometer, bioimpedance, ELISA, and polymerase chain reaction assays.
All investigated SNPs conformed to Hardy-Weinberg equilibrium with allele frequencies validated against CEU population. Genotype CC of rs7136446 associated with higher body fat and increased maximal force production. Genotype CC of the IGFBP3 SNP rs2854744 and TT genotype of the IL6R SNP rs4537545 associated with higher IL-6 levels. In logistic regression analysis, allele C of the rs2854744 decreased odds for lower body fat. None of the studied SNPs associated with aerobic performance.
Our data suggest that common variation in the IGF1 gene may affect maximal force production, which can be explained by the role of IGF-1 in the anabolic pathways of muscle and neurotrophy. Variations in the IGF1 and IGFBP3 gene may result in higher body fat and be related to alterations of IGF-1-mediated tissue growth.
我们阐明了胰岛素样生长因子-1（IGF1）、IGF 结合蛋白-3（IGFBP3）、白细胞介素-6（IL6）及其受体（IL6R）基因变异对肌肉和有氧性能、身体成分以及循环 IGF-1 和 IL-6 水平的影响。单核苷酸多态性（SNP）通常可能影响基因调控或表达，或相应蛋白的结构和功能，并改变其生物学效应。IGF-1 参与骨骼肌的合成代谢途径。IL-6 在剧烈体力活动期间肌肉能量平衡中起着重要作用。
841 名健康的芬兰白人男性受试者为高加索人，他们的 IGF1（rs6220 和 rs7136446）、IGFBP3（rs2854744）、IL6（rs1800795）和 IL6R（rs4537545）SNP 进行了基因分型，并研究了与腿部伸肌最大力、最大耗氧量、体脂百分比以及 IGF-1 和 IL-6 水平的相关性。分析方法包括测力计、自行车测力计、生物阻抗、ELISA 和聚合酶链反应检测。
所有研究的 SNP 均符合 Hardy-Weinberg 平衡，等位基因频率与 CEU 人群相对应。rs7136446 的 CC 基因型与体脂增加和最大力产生增加有关。IGFBP3 SNP rs2854744 的 CC 基因型和 IL6R SNP rs4537545 的 TT 基因型与较高的 IL-6 水平相关。在逻辑回归分析中，rs2854744 的等位基因 C 降低了体脂较低的几率。研究的 SNP 均与有氧性能无关。
我们的数据表明，IGF1 基因的常见变异可能会影响最大力产生，这可以用 IGF-1 在肌肉和神经营养的合成代谢途径中的作用来解释。IGF1 和 IGFBP3 基因的变异可能导致体脂增加，并与 IGF-1 介导的组织生长变化有关。
