同源 DNA 模板化学及其在核酸传感中的应用。
Homo-DNA templated chemistry and its application to nucleic acid sensing.
机构信息
Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern, Switzerland.
出版信息
Chem Commun (Camb). 2011 Jul 14;47(26):7494-6. doi: 10.1039/c1cc11469g. Epub 2011 May 9.
PMID:21552601
Abstract
We have investigated the homo-DNA templated Staudinger reduction of the profluorophore rhodamine azide and have applied this reaction to the detection of natural DNA with a hybrid homo-DNA/DNA molecular beacon. In this system the sensing and the reporting unit are bioorthogonal to each other which facilitates sequence design and increases fidelity.
摘要
我们研究了同系 DNA 模板化的 Staudinger 还原反应对前荧光素罗丹明叠氮化物的作用，并将该反应应用于具有杂交同系 DNA/DNA 分子信标的天然 DNA 的检测。在该系统中，传感和报告单元彼此为生物正交的，这有利于序列设计并提高了保真度。
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