基于靶标诱导 DNA 构象开关增强荧光偏振的钾离子超灵敏检测
Ultrasensitive detection of potassium ions based on target induced DNA conformational switch enhanced fluorescence polarization.
机构信息
Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, 541004, China.
出版信息
Analyst. 2012 Jun 21;137(12):2770-3. doi: 10.1039/c2an35416k. Epub 2012 May 3.
We have developed a simple, highly sensitive and selective fluorescence polarization assay for the detection of potassium ions based on target induced DNA conformational switch from hairpin to G-quadruplex enhanced fluorescence polarization. The assay was applied in the detection of low nM concentrations of potassium ions and was highly selective over other cations.
我们开发了一种简单、高灵敏、高选择性的荧光偏振检测钾离子的方法，该方法基于目标诱导的 DNA 构象从发夹到 G-四链体的转变，从而增强了荧光偏振。该方法可用于检测低纳摩尔浓度的钾离子，并且对其他阳离子具有高度选择性。
Zotero 插件