Institute of Health Research, São Paulo, SP, Brazil.
J Appl Oral Sci. 2011 Apr;19(2):125-9. doi: 10.1590/s1678-77572011000200008.
Human HOX genes encode transcription factors that act as master regulators of embryonic development. They are important in several processes such as cellular morphogenesis and differentiation. The HOXB5 gene in particular has been reported in some types of neoplasm, but not in oral cancer.
The present study investigated the expression of HOXB5 in oral squamous cell carcinoma (SCC) and in non-tumoral adjacent tissues, focusing on verifying its possible role as a broad tumor-associated gene and its association with histopathological and clinical (TNM) characteristics.
RT-PCR was performed to amplify HOXB5 mRNA in 15 OSCCs and adjacent non-tumoral epithelium. A possible association with TNM and histopathologic data was verified by the chi-square and post-hoc t-test.
HOXB5 was amplified in 60% non-tumoral epithelium and in 93.3% carcinomas. No statistically significant differences were found regarding the HOXB5 mRNA expression and TNM or histological grade.
HOXB5 is expressed in OSCCs and its role in cancer progression should be further investigated.
人类 HOX 基因编码转录因子，作为胚胎发育的主要调控因子。它们在细胞形态发生和分化等多个过程中发挥重要作用。HOXB5 基因尤其在某些类型的肿瘤中已有报道，但在口腔癌中尚未发现。
本研究调查了 HOXB5 在口腔鳞状细胞癌（SCC）和非肿瘤相邻组织中的表达，重点验证其作为广谱肿瘤相关基因的可能作用及其与组织病理学和临床（TNM）特征的关联。
通过 RT-PCR 扩增 15 例 OSCC 和相邻非肿瘤上皮中的 HOXB5 mRNA。通过卡方检验和事后 t 检验验证与 TNM 和组织病理学数据的可能关联。
HOXB5 在 60%的非肿瘤上皮和 93.3%的癌组织中扩增。HOXB5 mRNA 表达与 TNM 或组织学分级之间无统计学显著差异。
HOXB5 在 OSCC 中表达，其在癌症进展中的作用有待进一步研究。
