Department of Genetics, Osmania University, Hyderabad 500007, Andhra Pradesh, India.
Singapore Med J. 2011 Apr;52(4):283-8.
Oxidative and nitrosative stress caused by a disturbance in the homeostasis of pro-oxidants and antioxidants play a vital role in the pathogenesis of coronary heart disease (CHD). Enhanced formation of reactive oxygen species/reactive nitrogen species may also affect the oxidation/nitration of biomolecules such as lipids, proteins and DNA. The present study was undertaken to estimate oxidative and nitrosative stress, and to evaluate oxidative DNA damage.
The study population consisted of 120 patients with angiographically documented CHD and an equal number of age- and gender-matched healthy controls. Lipid profiles were estimated using Glaxo kits. Estimation of plasma malondialdehyde (MDA), nitrite/nitrate and comet assay were carried out using previously published methods.
Lipid profiles were significantly different in patients with coronary artery disease compared to the controls (p-value less than 0.01). The levels of MDA, nitrite/nitrate and DNA damage in the patients were significantly higher compared to the controls, and a strong correlation was found between the comet tail length and the MDA and nitrite/nitrate levels. Further analysis revealed that the influence of nitrite/nitrate was greater than that of MDA.
Our results indicate that abnormal levels of lipid profiles, along with increased oxidative and nitrosative stress as well as somatic DNA damage, could be important pathogenic factors that act as additional prognostic predictors. They may also serve as potential targets for therapeutic strategies in CHD for early management and prevention of the disease.
由于氧化剂和抗氧化剂之间的平衡失调导致的氧化应激和硝化应激在冠心病（CHD）的发病机制中起着至关重要的作用。活性氧/活性氮物质的形成增加也可能影响脂质、蛋白质和 DNA 等生物分子的氧化/硝化。本研究旨在评估氧化应激和硝化应激，并评估氧化 DNA 损伤。
研究人群包括 120 名经血管造影证实的 CHD 患者和年龄、性别匹配的 120 名健康对照者。使用 Glaxo 试剂盒估计血脂谱。使用先前发表的方法估计血浆丙二醛（MDA）、亚硝酸盐/硝酸盐和彗星试验。
与对照组相比，冠心病患者的血脂谱明显不同（p 值<0.01）。与对照组相比，患者的 MDA、亚硝酸盐/硝酸盐和 DNA 损伤水平明显更高，并且彗星尾长与 MDA 和亚硝酸盐/硝酸盐水平之间存在很强的相关性。进一步的分析表明，硝酸盐/硝酸盐的影响大于 MDA。
我们的结果表明，异常的血脂谱水平以及氧化应激和硝化应激的增加以及体细胞 DNA 损伤可能是重要的致病因素，作为额外的预后预测指标。它们也可能成为 CHD 早期管理和预防疾病的潜在治疗靶点。
