Kiaris H, Spandidos D
NATL HELLEN RES FND,INST BIOL RES & BIOTECHNOL,GR-16635 ATHENS,GREECE. UNIV CRETE,SCH MED,IRAKLION,GREECE.
Int J Oncol. 1995 Jul;7(1):75-80. doi: 10.3892/ijo.7.1.75.
The levels of expression of the ras family genes, in 14 tumour specimens from squamous cell carcinomas of the larynx were analysed by reverse transcription-polymerase chain reaction. The H-ras was overexpressed in 12 (86%) samples, K-ras in 11 (78%) and N-ras in 8 (57%) samples. All tumours exhibited overexpression of at least one member of the I-as family. In addition, each member of the ras family was activated independently from the rest of the ras family genes. The incidence of amplification in the ms family genes was also analysed by differential PCR: K-rns was found amplified in 14% (2/14), N-ras in 7% (1/14) and H-ras in none of the samples tested. Amplification data exhibited no association with the expressional levels of the ras genes. Furthermore, we investigated the incidence of codon 12 point mutations in the ras family genes but no mutation was found. The present study indicates that overexpression of the ras family genes is important for the development of the disease and it is not associated with the amplification status of the genes. In addition, the differential regulation among the members of the ms family might play a role in the development of laryngeal tumours.
通过逆转录-聚合酶链反应分析了14例喉鳞状细胞癌肿瘤标本中ras家族基因的表达水平。12例（86%）样本中H-ras过表达，11例（78%）样本中K-ras过表达，8例（57%）样本中N-ras过表达。所有肿瘤均表现出ras家族至少一个成员的过表达。此外，ras家族的每个成员都是独立于ras家族其他基因被激活的。还通过差异PCR分析了ras家族基因的扩增发生率：发现14%（2/14）的样本中K-ras扩增，7%（1/14）的样本中N-ras扩增，而在所检测的样本中未发现H-ras扩增。扩增数据与ras基因的表达水平无关联。此外，我们研究了ras家族基因密码子12点突变的发生率，但未发现突变。本研究表明，ras家族基因的过表达对该疾病的发展很重要，且与基因的扩增状态无关。此外，ras家族成员之间的差异调节可能在喉肿瘤的发展中起作用。
