Institute of Biophysics, The Second Military Medical University, Shanghai, 200433, People's Republic of China.
Biol Trace Elem Res. 2011 Dec;144(1-3):183-96. doi: 10.1007/s12011-011-9064-3. Epub 2011 May 7.
Titanium dioxide (TiO(2)) nanoparticles (NPs) are massively fabricated and widely used in daily life, and thus potential risk has been posed to human health. However, the mechanism of the interaction between TiO(2) NPs and cells is still unclear. In this study, the interaction of anatase TiO(2) NPs with HaCaT cells is studied in vitro with multi-techniques. The TiO(2) NPs not only insert into cells through endocytic pathway but also penetrate into the cell. The TiO(2) NPs could produce reactive oxygen species (ROS) after dispersion spontaneously. Furthermore, the interaction between TiO(2) NPs and cellular components might also generate ROS. The ROS generation could lead to cellular toxicity if the level of ROS production overwhelms the antioxidant defense. Cytoskeletal components, particularly the microfilaments and microtubules, cause modifications upon exposure to TiO(2) NPs. With all results, the toxicological effects of TiO(2) NPs on HaCaT cell can be simplified into six events.
二氧化钛（TiO(2)）纳米颗粒（NPs）被大规模制造并广泛应用于日常生活中，因此对人类健康构成了潜在风险。然而，TiO(2) NPs 与细胞相互作用的机制仍不清楚。在这项研究中，使用多种技术研究了锐钛矿 TiO(2) NPs 与 HaCaT 细胞的体外相互作用。TiO(2) NPs 不仅通过内吞途径插入细胞，还穿透细胞。TiO(2) NPs 分散后会自发产生活性氧（ROS）。此外，TiO(2) NPs 与细胞成分的相互作用也可能产生 ROS。如果 ROS 的产生水平超过抗氧化防御，ROS 的产生可能导致细胞毒性。细胞骨架成分，特别是微丝和微管，在暴露于 TiO(2) NPs 后会发生修饰。综上所述，TiO(2) NPs 对 HaCaT 细胞的毒理学影响可以简化为六个事件。
