Nica Ionela Cristina, Miu Bogdan Andrei, Stan Miruna S, Diamandescu Lucian, Dinischiotu Anca
Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania.
Research Institute of the University of Bucharest-ICUB, University of Bucharest, 050657 Bucharest, Romania.
Nanomaterials (Basel). 2022 Feb 25;12(5):770. doi: 10.3390/nano12050770.
Titanium dioxide nanoparticles (TiO NPs) are found in several products on the market that include paints, smart textiles, cosmetics and food products. Besides these, TiO NPs are intensively researched for their use in biomedicine, agriculture or installations to produce energy. Taking into account that several risks have been associated with the use of TiO NPs, our aim was to provide TiO NPs with improved qualities and lower toxicity to humans and the environment. Pure TiO P25 NPs and the same NPs co-doped with iron (1%) and nitrogen atoms (P25-Fe(1%)-N NPs) by hydrothermal treatment to increase the photocatalytic activity in the visible light spectrum were in vitro evaluated in the presence of human lung cells. After 24 and 72 h of incubation, the oxidative stress was initiated in a time- and dose-dependent manner with major differences between pure P25 and P25-Fe(1%)-N NPs as revealed by malondialdehyde and reactive oxygen species levels. Additionally, a lower dynamic of autophagic vacuoles formation was observed in cells exposed to Fe-N-doped P25 NPs compared to the pure ones. Therefore, our results suggest that Fe-N doping of TiO NPs can represent a valuable alternative to the conventional P25 Degussa particles in industrial and medical applications.
市场上的多种产品中都能发现二氧化钛纳米颗粒（TiO NPs），这些产品包括涂料、智能纺织品、化妆品和食品。除此之外，TiO NPs在生物医药、农业或能源生产装置中的应用也受到了广泛研究。鉴于TiO NPs的使用存在多种风险，我们的目标是制备出品质更优、对人类和环境毒性更低的TiO NPs。通过水热处理，制备了纯TiO P25 NPs以及共掺杂1%铁和氮原子的相同纳米颗粒（P25-Fe(1%)-N NPs），以提高其在可见光光谱中的光催化活性，并在人肺细胞存在的情况下进行了体外评估。孵育24小时和72小时后，丙二醛和活性氧水平显示，氧化应激以时间和剂量依赖的方式引发，纯P25 NPs和P25-Fe(1%)-N NPs之间存在显著差异。此外，与纯P25 NPs相比，暴露于铁氮共掺杂P25 NPs的细胞中自噬空泡形成的动态较低。因此，我们的结果表明，在工业和医学应用中，TiO NPs的铁氮掺杂可能是传统德固赛P25颗粒的一种有价值的替代品。
