在真菌构巢曲霉中，Hsp30、Hsp70 和 Hsp90 热休克蛋白基因的转录受 PalA 蛋白的调控，以响应酸 pH 感应。
Transcription of the Hsp30, Hsp70, and Hsp90 heat shock protein genes is modulated by the PalA protein in response to acid pH-sensing in the fungus Aspergillus nidulans.
机构信息
Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, 14049-900, Ribeirão Preto, São Paulo, Brazil.
出版信息
Cell Stress Chaperones. 2011 Sep;16(5):565-72. doi: 10.1007/s12192-011-0267-5. Epub 2011 May 8.
Abstract
Heat shock proteins are molecular chaperones linked to a myriad of physiological functions in both prokaryotes and eukaryotes. In this study, we show that the Aspergillus nidulans hsp30 (ANID_03555.1), hsp70 (ANID_05129.1), and hsp90 (ANID_08269.1) genes are preferentially expressed in an acidic milieu, whose expression is dependent on the palA (+) background under optimal temperature for fungal growth. Heat shock induction of these three hsp genes showed different patterns in response to extracellular pH changes in the palA(+) background. However, their accumulation upon heating for 2 h was almost unaffected by ambient pH changes in the palA (-) background. The PalA protein is a member of a conserved signaling cascade that is involved in the pH-mediated regulation of gene expression. Moreover, we identified several genes whose expression at pH 5.0 is also dependent on the palA (+) background. These results reveal novel aspects of the heat- and pH-sensing networks of A. nidulans.
摘要
热休克蛋白是分子伴侣，与原核生物和真核生物的许多生理功能有关。在这项研究中，我们表明，构巢曲霉 hsp30（ANID_03555.1）、hsp70（ANID_05129.1）和 hsp90（ANID_08269.1）基因在酸性环境中优先表达，其表达依赖于真菌生长最佳温度下的 palA（+）背景。在 palA（+）背景下，这三个 hsp 基因的热休克诱导表现出不同的对外界 pH 值变化的响应模式。然而，在 palA（-）背景下，它们在加热 2 小时后的积累几乎不受环境 pH 值变化的影响。PalA 蛋白是参与 pH 介导的基因表达调控的保守信号级联反应的成员。此外，我们还鉴定了几个在 pH 5.0 时表达也依赖于 palA（+）背景的基因。这些结果揭示了构巢曲霉热和 pH 感应网络的新方面。
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