Zhang Lan, Xiong Chen, Wu Yanzhao, Shi Huijing, Jing Shanghua, Song Junjian, Zhao Zhen, Shan Baoen
Department of Otolaryngology, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2012 Sep;26(18):798-801.
To study the correlation between the stromal cell-derived factor (SDF-1) and the receptor fusin (CXCR4) in carcinoma of larynx, and investigate some mechanisms of SDF-1/CXCR4 during the development, invasion and lymph node metastasis of laryngocarcinoma.
Detecting the expression of SDF-1 and CXCR4 by immunohistochemical method (SP) in laryngocarcinoma, paraneoplastic tissues, normal laryngeal mucosa and cervical lymph node. Using Kruskal-Wallis H test, chi2 test, Spearman rank correlation analysis and so on to do statistical analysis.
The positive expression rate of SDF-1 and CXCR4 in laryngocarcinoma was obviously higher than in paraneoplastic tissues and normal laryngeal mucosa tissues (P < 0.01). And the expression of two proteins was correlated with lymph node metastasis (P < 0.01), clinical stage (P < 0.01) and pathological grading of tumor (P < 0.05). The positive expression rate of SDF-1 and CXCR4 protein in metastasis lymph node tissue was higher than that in non metastasis lymph node tissue (P < 0.01). The expression of SDF-1 is correlated positively with the expression of CXCR4 in laryngocarcinoma.
SDF-1 and CXCR4 protein are highly expressed in laryngocarcinoma and in metastasis lymph node tissue. And they are correlated with lymph node metastasis, clinical stage and pathological grading of the tumor. According to the results, the two proteins may relate to infiltration and metastasis of laryngeal squamous cell carcinoma and play a role of synergistic action in the development and invasion of carcinoma of larynx.
研究喉癌组织中基质细胞衍生因子（SDF-1）及其受体融合素（CXCR4）的表达情况，探讨SDF-1/CXCR4在喉癌发生、发展、侵袭及淋巴结转移过程中的作用机制。
采用免疫组织化学SP法检测喉癌组织、癌旁组织、正常喉黏膜组织及颈部淋巴结中SDF-1和CXCR4的表达，应用Kruskal-Wallis H检验、χ2检验、Spearman等级相关分析等进行统计学分析。
喉癌组织中SDF-1和CXCR4的阳性表达率明显高于癌旁组织和正常喉黏膜组织（P<0.01）。二者的表达与喉癌的淋巴结转移（P<0.01）、临床分期（P<0.01）及肿瘤病理分级（P<0.05）相关。转移淋巴结组织中SDF-1和CXCR4蛋白的阳性表达率高于未转移淋巴结组织（P<0.01）。喉癌组织中SDF-1的表达与CXCR4的表达呈正相关。
SDF-1和CXCR4蛋白在喉癌组织及转移淋巴结组织中高表达，与喉癌的淋巴结转移、临床分期及肿瘤病理分级相关。提示二者可能参与喉鳞状细胞癌的浸润和转移，在喉癌的发生发展过程中起协同作用。
