Valensin P E, Bianchi Bandinelli M L, Zazzi M, Gaggelli E, Valensin G
Istituto di Microbiologia, Università di Siena, Italy.
Microbiologica. 1990 Jan;13(1):11-9.
The water proton Nuclear Magnetic Resonance (NMR) spin-lattice relaxation rates were measured in suspension of uninfected and Herpes simplex virus type 2-infected WI-38 diploid cells and shown to allow reliable detection of (i) the early signs of productive infection, and (ii) the tendency to undergo either spontaneous or virus-induced transformation. In addition, NMR investigation of several transformed cell lines currently and previously established from WI-38 cells clearly indicated that the NMR relaxation parameter is a suitable tool for assessment of the level of malignancy, as revealed by in vivo oncogenicity tests in immunosuppressed mice.
在未感染和感染2型单纯疱疹病毒的WI-38二倍体细胞悬液中测量了水质子核磁共振（NMR）自旋晶格弛豫率，结果表明该弛豫率能够可靠地检测出：（i） productive感染的早期迹象；（ii） 自发或病毒诱导转化的倾向。此外，对目前以及之前从WI-38细胞建立的几种转化细胞系进行的NMR研究清楚地表明，NMR弛豫参数是评估恶性程度的合适工具，这一点已通过免疫抑制小鼠体内致癌性试验得到证实。
