Department of Physiology, University of Massachusetts Medical Center, Worcester, Massachusetts, USA. Department of Anesthesiology, University of Massachusetts Medical Center, Worcester, Massachusetts, USA. Laboratory of Experimental Neurology, Neurology Service (127) - DVAMC, Nashville, Tennessee, USA.
J Neuroendocrinol. 1992 Apr;4(2):193-205. doi: 10.1111/j.1365-2826.1992.tb00159.x.
The present study was done to determine whether the vasopressinergic neurons in the hypothalamus controlling flank marking behavior are distinct from the magnocellular neurons comprising the hypothalamo-neurohypophysial system. Animals were either hypophysectomized or injected with a suicide transport lectin, volkensin, into the neurohypophysis. Both procedures resulted in a pronounced loss of vasopressin-immunoreactive perikarya throughout the hypothalamus concomitant with increases in water intake and urine output and decreases in circulating levels of vasopressin. The loss of the hypothalamo-neurohypophysial system was most pronounced in volkensin-treated animals that presented with frank diabetes insipidus and exceedingly low levels of plasma vasopressin. However, the vasopressinergic fibers and magnocellular neurons in and around the anterior hypothalamus implicated in the control of flank marking survived the volkensin treatment. Volkensin-treated animals exhibited levels of flank marking typical of untreated animals. These data suggest the presence of anatomically and functionally distinct populations of vasopressinergic magnocellular neurons in the hypothalamus of the golden hamster.
本研究旨在确定控制侧斑标记行为的下丘脑血管加压素能神经元是否与构成下丘脑-神经垂体系统的大细胞神经元不同。动物要么被垂体切除术，要么被自杀运输凝集素沃尔肯辛注射到神经垂体。这两种方法都导致了明显的血管加压素免疫反应性神经元胞体在整个下丘脑的丧失，伴随着饮水量和尿量的增加以及循环中血管加压素水平的降低。沃尔肯辛处理的动物表现出明显的下丘脑-神经垂体系统丧失，表现为明显的尿崩症和极低的血浆血管加压素水平。然而，在前下丘脑内和周围与控制侧斑标记有关的血管加压素能纤维和大细胞神经元仍然存在。沃尔肯辛处理的动物表现出与未处理动物相似的侧斑标记水平。这些数据表明，在金黄仓鼠的下丘脑存在解剖和功能上不同的血管加压素能大细胞神经元群体。
