Department of Laboratory Medicine, Eastern Hepatobiliary Hospital, Second Military Medical University, Shanghai 200438, People's Republic of China.
Mol Biol Rep. 2012 Feb;39(2):797-803. doi: 10.1007/s11033-011-0801-7. Epub 2011 May 10.
CXCL14 (C-X-C motif chemokine ligand 14) is a conserved member of chemokine family and functions as a chemoattractant for multiplicate immunocytes. CXCL14 expression is constitutive in normal tissues, but absent in wide range of epithelial tumors. Many reports have claimed its important role in tumorigenesis and vascularization. An association between rs2237062 polymorphism and hepatocellular carcinoma (HCC) susceptibility was found in patients with chronic HCV infection in Japanese population. Here we analyzed, by using a polymerase chain reaction-ligation detection reaction (PCR-LDR), the polymorphism in 202 non-HCC patients with HBV infection, 361 HBV-related HCC patients and 407 healthy controls. The aim was to detect the possible association of this single-nucleotide polymorphism (SNP) with HBV-related HCC susceptibility and progression. However, no association was found between rs2237062 polymorphism and susceptibility to HBV infection or HBV-related HCC. Intriguingly, our stratification analysis revealed that HBV-related HCC patients in advanced phase (TNM-II-IV stage) had significantly higher C allele frequency at this polymorphism than patients at early stage (TNM-I stage) (33.5% vs. 25.7%), and its odds ratio reached 1.47 (95% CI 1.06-2.04, P = 0.021). These results suggest that the rs2237062 polymorphism in the CXCL14 gene might influence HBV-related HCC progression in Chinese population.
细胞因子趋化因子配体 14（CXCL14）是趋化因子家族中的一个保守成员，作为多种免疫细胞的趋化因子发挥作用。CXCL14 在正常组织中持续表达，但在广泛的上皮肿瘤中缺失。许多报道声称其在肿瘤发生和血管生成中起重要作用。在日本慢性 HCV 感染患者中发现 rs2237062 多态性与肝细胞癌（HCC）易感性之间存在关联。在这里，我们通过聚合酶链反应-连接检测反应（PCR-LDR）分析了 202 例乙型肝炎病毒（HBV）感染非 HCC 患者、361 例 HBV 相关 HCC 患者和 407 名健康对照者中的多态性。目的是检测该单核苷酸多态性（SNP）与 HBV 相关 HCC 易感性和进展的可能关联。然而，未发现 rs2237062 多态性与 HBV 感染或 HBV 相关 HCC 的易感性相关。有趣的是，我们的分层分析表明，处于晚期（TNM-II-IV 期）的 HBV 相关 HCC 患者在该多态性中 C 等位基因频率明显高于早期（TNM-I 期）患者（33.5%比 25.7%），其比值比达到 1.47（95%CI 1.06-2.04，P=0.021）。这些结果表明，CXCL14 基因中的 rs2237062 多态性可能影响中国人群中 HBV 相关 HCC 的进展。
