Niu Jianjun, Lin Yong, Liu Pingguo, Yu Yiwen, Su Chenghao, Wang Xiaomin
Zhongshan Hospital, Medical College of Xiamen University, Xiamen, China.
Fujian Provincial Key Laboratory of Chronic Liver Disease and Hepatocellular Carcinoma, Xiamen, China.
Oncotarget. 2016 Nov 15;7(46):76169-76180. doi: 10.18632/oncotarget.12732.
Long non-coding RNAs are involved with development and progression of cancer, and the advance of microarray technology allows the researchers to investigate the complete expression profile of lncRNA in various kinds of sample. We enrolled 5 male primary HCC cases with chronic HBV infection and the HCC and normal tissues have been obtained during the resection surgery. After total RNA extraction, the lncRNA microarray analysis was conducted to determine the lncRNA and mRNA expression signals. 612 lncRNAs and 1,064 mRNAs were significantly up-regulated in HCC tissue while 656 lncRNAs and 1,532 mRNAs were down-regulated in HCC tissues. Compared with normal tissues, XLOC_007433 (fold change: 12.80) and AC144449.1 (fold change: 27.20) were the most over- and under-expressed lncRNAs in HCC tissues. As for the mRNA, THBS4 (fold change:41.13) and CXCL14 (fold change: 58.03) were the most over- and under-expressed mRNAs in HCC tissues when comparing with their normal counterparts. In total, 4,552 pairs of lncRNA-mRNA were identified and the co-expression network was constructed. Moreover, the gene ontology enrichment analysis showed that the significantly different transcript between HCC and normal tissues were mainly associated with response to wounding, inflammatory response, protein hetrodimerization activity, response to stress which involved with biological process and molecular function. The pathway analysis suggested that the most significant pathways consisted of alcoholism, regulatory RNA pathways and RNA polymerase transcription. Several novel differentially expressed lncRNAs and mRNAs were identified in the present study.
长链非编码RNA与癌症的发生发展相关，而微阵列技术的进步使研究人员能够研究lncRNA在各种样本中的完整表达谱。我们纳入了5例慢性HBV感染的男性原发性肝癌病例，并在切除手术中获取了肝癌组织和正常组织。提取总RNA后，进行lncRNA微阵列分析以确定lncRNA和mRNA的表达信号。在肝癌组织中，612个lncRNA和1,064个mRNA显著上调，而656个lncRNA和1,532个mRNA下调。与正常组织相比，XLOC_007433（倍数变化：12.80）和AC144449.1（倍数变化：27.20）是肝癌组织中表达上调和下调最明显的lncRNA。至于mRNA，与正常对应物相比，THBS4（倍数变化：41.13）和CXCL14（倍数变化：58.03）是肝癌组织中表达上调和下调最明显的mRNA。总共鉴定出4,552对lncRNA-mRNA并构建了共表达网络。此外，基因本体富集分析表明，肝癌组织与正常组织之间显著不同的转录本主要与伤口反应、炎症反应、蛋白质异二聚化活性、应激反应相关，这些涉及生物学过程和分子功能。通路分析表明，最显著的通路包括酒精中毒、调控RNA通路和RNA聚合酶转录。本研究鉴定出了几种新的差异表达lncRNA和mRNA。
