Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, China.
Infect Genet Evol. 2011 Dec;11(8):2083-8. doi: 10.1016/j.meegid.2011.09.025. Epub 2011 Oct 12.
Interleukin-16 (IL16) as a multifunctional cytokine, plays a key role in inflammatory and autoimmune diseases as well as tumour growth and progression. Recently, genetic polymorphisms of IL16 have been reported to be associated with susceptibility to a range of cancers. This study was undertaken to investigate the IL16 gene polymorphisms and determine whether these genetic factors are related to the occurrence of hepatocellular carcinoma (HCC) in a Chinese population.
We analyzed three polymorphisms of the IL16 gene (rs11556218T/G, rs4072111C/T and rs4778889T/C) in 206 patients with HBV-related HCC, 270 chronic hepatitis B patients and 264 healthy controls, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and DNA sequencing technology.
IL16 polymorphisms were not associated with risk of HCC when compared with healthy controls. However, IL16 polymorphisms were significantly associated with susceptibility to HBV-related HCC when using chronic hepatitis B patients as controls. The rs11556218T/G TG and GG genotypes were associated with significantly increased risk of HBV-related HCC compared with the TT genotype (OR = 1.96 and OR = 3.33). The data also revealed that subjects with the G allele appeared to have higher susceptibility to HBV-related HCC than those with the T allele (OR = 2.10). Under the dominant model genotype TG+GG appeared to be associated with an increased risk of HBV-related HCC (OR = 2.18). The rs4072111C/T TT genotype was associated with a significantly increased risk of HBV-related HCC compared with the CC genotype (OR = 6.67). Polymorphisms of the IL16 gene were significantly associated with susceptibility to chronic hepatitis B when using healthy subjects as controls. The rs11556218T/G TG and GG genotypes were associated with significantly decreased risk of chronic hepatitis B compared with the TT genotype (OR = 0.49 and OR = 0.29). The data also revealed that subjects with the G allele appeared to have lower susceptibility to chronic hepatitis B than those with the T allele (OR = 0.46). Under the dominant model genotype TG + GG appeared to have lower susceptibility to chronic hepatitis B (OR = 0.44).
This study showed that the genotypes and allele of IL16 SNPs were associated with chronic HBV infection and HCC. However, further investigation with a larger sample size and haplotype analysis with other SNPs may be required to validate the genetic effects of the IL16 polymorphisms on chronic HBV infection and HCC.
白细胞介素 16（IL16）作为一种多功能细胞因子，在炎症和自身免疫性疾病以及肿瘤生长和进展中发挥关键作用。最近，IL16 的基因多态性已被报道与多种癌症的易感性有关。本研究旨在探讨 IL16 基因多态性，确定这些遗传因素是否与中国人群肝细胞癌（HCC）的发生有关。
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）法和 DNA 测序技术，分析 206 例乙型肝炎病毒（HBV）相关 HCC 患者、270 例慢性乙型肝炎患者和 264 例健康对照者的 IL16 基因三个多态性（rs11556218T/G、rs4072111C/T 和 rs4778889T/C）。
与健康对照组相比，IL16 多态性与 HCC 风险无相关性。然而，当以慢性乙型肝炎患者为对照时，IL16 多态性与 HBV 相关 HCC 的易感性显著相关。与 TT 基因型相比，rs11556218T/G 的 TG 和 GG 基因型与 HBV 相关 HCC 的风险显著增加（OR=1.96 和 OR=3.33）。数据还显示，与 T 等位基因相比，G 等位基因的个体似乎更容易发生 HBV 相关 HCC（OR=2.10）。在显性模型中，TG+GG 基因型似乎与 HBV 相关 HCC 的风险增加相关（OR=2.18）。与 CC 基因型相比，rs4072111C/T 的 TT 基因型与 HBV 相关 HCC 的风险显著增加（OR=6.67）。当以健康受试者为对照时，IL16 基因多态性与慢性乙型肝炎的易感性显著相关。与 TT 基因型相比，rs11556218T/G 的 TG 和 GG 基因型与慢性乙型肝炎的风险显著降低（OR=0.49 和 OR=0.29）。数据还显示，与 T 等位基因相比，G 等位基因的个体似乎更容易发生慢性乙型肝炎（OR=0.46）。在显性模型中，TG+GG 基因型似乎对慢性乙型肝炎的易感性降低（OR=0.44）。
本研究表明，IL16 SNP 的基因型和等位基因与慢性 HBV 感染和 HCC 相关。然而，需要进一步进行更大样本量的研究和其他 SNP 的单体型分析，以验证 IL16 多态性对慢性 HBV 感染和 HCC 的遗传影响。
