脊髓 5-羟色胺受体在电针对炎性痛大鼠镇痛中的作用。
Involvement of spinal serotonin receptors in electroacupuncture anti-hyperalgesia in an inflammatory pain rat model.
机构信息
Center for Integrative Medicine, School of Medicine, University of Maryland, 685 W Baltimore street, MSTF Rm 8-22, Baltimore, MD 21201, USA.
出版信息
Neurochem Res. 2011 Oct;36(10):1785-92. doi: 10.1007/s11064-011-0495-1. Epub 2011 May 10.
Abstract
We previously showed that electroacupuncture (EA) activates medulla-spinal serotonin-containing neurons. The present study investigated the effects of intrathecal 5,7-dihydroxytryptamine creatinine sulfate, a selective neurotoxin for serotonergic terminals, the 5-hydroxytryptamine 1A receptor (5-HT1AR) antagonist NAN-190 hydrobromide and the 5-HT2C receptor (5-HT2CR) antagonist SB-242,084 on EA anti-hyperalgesia. EA was given twice at acupoint GB30 after complete Freund's adjuvant (CFA) injection into hind paw. CFA-induced hyperalgesia was measured by assessing hind paw withdrawal latency (PWL) to a noxious thermal stimulus 30 min post-EA. Serotonin depletion and the 5-HT1AR antagonist blocked EA anti-hyperalgesia; the 5-HT2CR antagonist did not. Immunohistochemical staining showed that spinal 5-HT1AR was expressed and that 5-HT2CR was absent in naive and CFA-injected animals 2.5 h post-CFA. These results show a correlation between EA anti-hyperalgesia and receptor expression. Collectively, the data show that EA activates supraspinal serotonin neurons to release 5-HT, which acts on spinal 5-HT1AR to inhibit hyperalgesia.
摘要
我们之前的研究表明，电针（EA）可以激活含有脊髓血清素的神经元。本研究旨在探讨鞘内给予 5,7-二羟基色胺肌酸硫酸盐（一种选择性的血清素末端神经毒素）、5-羟色胺 1A 受体（5-HT1AR）拮抗剂 NAN-190 氢溴酸盐和 5-羟色胺 2C 受体（5-HT2CR）拮抗剂 SB-242,084 对 EA 抗痛觉过敏的影响。在向足底注射完全弗氏佐剂（CFA）后，在穴位 GB30 处给予两次 EA。通过评估 EA 后 30 分钟对有害热刺激的后足撤回潜伏期（PWL）来测量 CFA 诱导的痛觉过敏。血清素耗竭和 5-HT1AR 拮抗剂阻断了 EA 抗痛觉过敏；5-HT2CR 拮抗剂则没有。免疫组织化学染色显示，在 CFA 注射后 2.5 小时，脊髓 5-HT1AR 表达，而 5-HT2CR 在未处理和 CFA 注射动物中均不存在。这些结果表明 EA 抗痛觉过敏与受体表达之间存在相关性。总的来说，这些数据表明，EA 激活了中枢血清素神经元以释放 5-HT，5-HT 作用于脊髓 5-HT1AR 以抑制痛觉过敏。
相似文献
1
Involvement of spinal serotonin receptors in electroacupuncture anti-hyperalgesia in an inflammatory pain rat model.脊髓 5-羟色胺受体在电针对炎性痛大鼠镇痛中的作用。
Neurochem Res. 2011 Oct;36(10):1785-92. doi: 10.1007/s11064-011-0495-1. Epub 2011 May 10.
2
Electroacupuncture inhibition of hyperalgesia in an inflammatory pain rat model: involvement of distinct spinal serotonin and norepinephrine receptor subtypes.电针对炎症痛大鼠模型痛觉过敏的抑制作用：涉及不同脊髓 5-羟色胺和去甲肾上腺素受体亚型。
Br J Anaesth. 2012 Aug;109(2):245-52. doi: 10.1093/bja/aes136. Epub 2012 May 23.
3
Spinal Serotonin 1A Receptor Contributes to the Analgesia of Acupoint Catgut Embedding by Inhibiting Phosphorylation of the N-Methyl-d-Aspartate Receptor GluN1 Subunit in Complete Freund's Adjuvant-Induced Inflammatory Pain in Rats.脊髓 5-羟色胺 1A 受体通过抑制完全弗氏佐剂诱导的炎症性疼痛大鼠 NMDA 受体 GluN1 亚单位磷酸化促进穴位埋线的镇痛作用。
J Pain. 2019 Jan;20(1):16.e1-16.e16. doi: 10.1016/j.jpain.2018.07.011. Epub 2018 Aug 10.
4
Rostral ventromedial medulla μ, but not κ, opioid receptors are involved in electroacupuncture anti-hyperalgesia in an inflammatory pain rat model.电针对炎症痛大鼠镇痛中延髓腹内侧头端 μ 阿片受体，而非 κ 阿片受体的激活作用。
Brain Res. 2011 Jun 13;1395:38-45. doi: 10.1016/j.brainres.2011.04.037. Epub 2011 Apr 28.
5
Involvement of opioid receptors in electroacupuncture-produced anti-hyperalgesia in rats with peripheral inflammation.阿片受体在电针减轻外周炎症大鼠痛觉过敏中的作用。
Brain Res. 2004 Sep 10;1020(1-2):12-7. doi: 10.1016/j.brainres.2004.05.067.
6
A parametric study of electroacupuncture on persistent hyperalgesia and Fos protein expression in rats.电针对大鼠持续性痛觉过敏和Fos蛋白表达的参数研究。
Brain Res. 2004 Sep 10;1020(1-2):18-29. doi: 10.1016/j.brainres.2004.01.092.
7
Effects of pertussis toxin on electroacupuncture-produced anti-hyperalgesia in inflamed rats.百日咳毒素对电针诱导的炎症大鼠抗痛觉过敏的影响。
Brain Res. 2005 May 17;1044(1):87-92. doi: 10.1016/j.brainres.2005.03.006. Epub 2005 Apr 1.
8
Electroacupuncture combined with MK-801 prolongs anti-hyperalgesia in rats with peripheral inflammation.电针联合MK-801可延长外周炎症大鼠的抗痛觉过敏时间。
Pharmacol Biochem Behav. 2005 May;81(1):146-51. doi: 10.1016/j.pbb.2005.03.002. Epub 2005 Apr 25.
9
The Effects of Electroacupuncture on the Apelin/APJ System in the Spinal Cord of Rats With Inflammatory Pain.电针对炎性疼痛大鼠脊髓中阿片肽/阿片肽受体系统的影响
Anesth Analg. 2016 Dec;123(6):1603-1610. doi: 10.1213/ANE.0000000000001535.
10
Involvement of nociceptin/orphanin FQ and its receptor in electroacupuncture-produced anti-hyperalgesia in rats with peripheral inflammation.孤啡肽及其受体在电针对外周炎症大鼠产生的抗痛觉过敏中的作用
Brain Res. 2006 Mar 17;1078(1):212-8. doi: 10.1016/j.brainres.2006.01.026. Epub 2006 Mar 24.
引用本文的文献
1
Adolescent ethanol drinking promotes hyperalgesia, neuroinflammation and serotonergic deficits in mice that persist into adulthood.青少年期乙醇摄入会促进小鼠的痛觉过敏、神经炎症和 5-羟色胺能缺陷，这些异常会持续到成年期。
Brain Behav Immun. 2023 Jan;107:419-431. doi: 10.1016/j.bbi.2022.07.160. Epub 2022 Jul 28.
2
Efficacy of Electroacupuncture for the Treatment of Postherpetic Neuralgia: Study Protocol for a Multicenter Randomized Controlled Trial.电针治疗带状疱疹后神经痛的疗效：一项多中心随机对照试验的研究方案
J Pain Res. 2022 Apr 5;15:959-968. doi: 10.2147/JPR.S357435. eCollection 2022.
3
Role of Neuroimmune Crosstalk in Mediating the Anti-inflammatory and Analgesic Effects of Acupuncture on Inflammatory Pain.神经免疫相互作用在介导针刺对炎性疼痛的抗炎和镇痛作用中的作用
Front Neurosci. 2021 Aug 2;15:695670. doi: 10.3389/fnins.2021.695670. eCollection 2021.
4
The Role of Neuroglial Crosstalk and Synaptic Plasticity-Mediated Central Sensitization in Acupuncture Analgesia.神经胶质细胞串扰和突触可塑性介导的中枢敏化在针刺镇痛中的作用
Neural Plast. 2021 Jan 18;2021:8881557. doi: 10.1155/2021/8881557. eCollection 2021.
5
The Involvement of Descending Pain Inhibitory System in Electroacupuncture-Induced Analgesia.下行痛觉抑制系统在电针镇痛中的作用
Front Integr Neurosci. 2019 Aug 21;13:38. doi: 10.3389/fnint.2019.00038. eCollection 2019.
6
The preemptive analgesia of pre-electroacupuncture in rats with formalin-induced acute inflammatory pain.电针预处理对福尔马林诱导的急性炎性痛大鼠的超前镇痛作用。
Mol Pain. 2019 Jan-Dec;15:1744806919866529. doi: 10.1177/1744806919866529.
7
Auricular vagus nerve stimulation enhances central serotonergic function and inhibits diabetic neuropathy development in Zucker fatty rats.耳迷走神经刺激增强中枢 5-羟色胺能功能并抑制 Zucker 肥胖大鼠糖尿病神经病变的发展。
Mol Pain. 2018 Jan-Dec;14:1744806918787368. doi: 10.1177/1744806918787368. Epub 2018 Jun 19.
8
Response to acupuncture treatment in horses with chronic laminitis.慢性蹄叶炎马匹对针灸治疗的反应。
Can Vet J. 2017 Aug;58(8):823-827.
9
Electroacupuncture Pretreatment at GB20 Exerts Antinociceptive Effects via Peripheral and Central Serotonin Mechanism in Conscious Migraine Rats.电针刺激足少阳胆经20穴预处理通过外周和中枢5-羟色胺机制对清醒偏头痛大鼠发挥抗伤害感受作用。
Evid Based Complement Alternat Med. 2016;2016:1846296. doi: 10.1155/2016/1846296. Epub 2016 Oct 24.
10
Molecular mechanisms underlying the effects of acupuncture on neuropathic pain.针刺治疗神经性疼痛作用的分子机制。
Neural Regen Res. 2013 Sep 5;8(25):2350-9. doi: 10.3969/j.issn.1673-5374.2013.25.006.
本文引用的文献
1
Effects of 5-hydroxytryptamine on substantia gelatinosa neurons of the trigeminal subnucleus caudalis in immature mice.5-羟色胺对未成年小鼠三叉神经尾核亚核胶状质神经元的影响。
Brain Res. 2011 Jan 12;1368:91-101. doi: 10.1016/j.brainres.2010.10.050. Epub 2010 Nov 26.
2
Contralateral electroacupuncture pretreatment suppresses carrageenan-induced inflammatory pain via the opioid-mu receptor.对侧电针预处理通过阿片 μ 受体抑制角叉菜胶诱导的炎性疼痛。
Rheumatol Int. 2011 Jun;31(6):725-30. doi: 10.1007/s00296-010-1364-y. Epub 2010 Feb 4.
3
Responses to 5-HT in morphologically identified neurons in the rat substantia gelatinosa in vitro.大鼠脊髓背角胶状质中形态学鉴定神经元对5-羟色胺的反应（体外研究）
Neuroscience. 2009 Mar 3;159(1):316-24. doi: 10.1016/j.neuroscience.2008.12.021. Epub 2008 Dec 24.
4
Neural mechanism underlying acupuncture analgesia.针刺镇痛的神经机制。
Prog Neurobiol. 2008 Aug;85(4):355-75. doi: 10.1016/j.pneurobio.2008.05.004. Epub 2008 Jun 5.
5
Electroacupuncture suppresses hyperalgesia and spinal Fos expression by activating the descending inhibitory system.电针通过激活下行抑制系统来抑制痛觉过敏和脊髓Fos表达。
Brain Res. 2007 Dec;1186:171-9. doi: 10.1016/j.brainres.2007.10.022. Epub 2007 Oct 22.
6
Release of GABA and activation of GABA(A) in the spinal cord mediates the effects of TENS in rats.脊髓中γ-氨基丁酸（GABA）的释放及GABA（A）的激活介导了经皮电刺激神经疗法（TENS）对大鼠的作用。
Brain Res. 2007 Mar 9;1136(1):43-50. doi: 10.1016/j.brainres.2006.11.061. Epub 2007 Jan 16.
7
Electroacupuncture at ST-36 accelerates colonic motility and transit in freely moving conscious rats.电针足三里可促进自由活动的清醒大鼠的结肠运动和运输。
Am J Physiol Gastrointest Liver Physiol. 2006 Feb;290(2):G285-92. doi: 10.1152/ajpgi.00068.2005. Epub 2005 Oct 27.
8
Analgesic effect of electroacupuncture on inflammatory pain in the rat model of collagen-induced arthritis: mediation by cholinergic and serotonergic receptors.电针对胶原诱导性关节炎大鼠模型炎性疼痛的镇痛作用：胆碱能和5-羟色胺能受体的介导作用
Brain Res. 2005 Sep 28;1057(1-2):181-5. doi: 10.1016/j.brainres.2005.07.014.
9
Effects of electroacupuncture on cold allodynia in a rat model of neuropathic pain: mediation by spinal adrenergic and serotonergic receptors.电针对神经性疼痛大鼠模型冷痛觉过敏的影响：脊髓肾上腺素能和血清素能受体的介导作用
Exp Neurol. 2005 Oct;195(2):430-6. doi: 10.1016/j.expneurol.2005.06.018.
10
Effects of the high-efficacy 5-HT1A receptor agonist, F 13640 in the formalin pain model: a c-Fos study.高效5-HT1A受体激动剂F 13640在福尔马林疼痛模型中的作用：一项c-Fos研究。
Eur J Pharmacol. 2005 May 9;514(2-3):121-30. doi: 10.1016/j.ejphar.2005.03.016.
Zotero 插件