Field Yair, Sharon Eilon, Segal Eran
Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot, 76100, Israel,
Subcell Biochem. 2011;52:193-204. doi: 10.1007/978-90-481-9069-0_9.
Binding of transcription factors to functional sites is a fundamental step in transcriptional regulation. In this chapter, we discuss how transcription factors are thought to achieve specificity to their functional targets, despite their typically low concentrations and degenerate binding specificities, and the fact that in large genomes their functional binding sites must compete with their widespread alternative binding sites. We highlight the importance of the chromatin structure context of the binding sites in this process, and its dependency on the genomic DNA sequence.
转录因子与功能位点的结合是转录调控的基本步骤。在本章中，我们将讨论尽管转录因子通常浓度较低且结合特异性存在简并性，以及在大型基因组中其功能结合位点必须与其广泛存在的替代结合位点竞争，但转录因子如何实现对其功能靶点的特异性识别。我们强调了结合位点的染色质结构背景在这一过程中的重要性，以及它对基因组DNA序列的依赖性。
