Department of General Biochemistry, Institute of Biochemistry, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
Platelets. 2011;22(4):277-83. doi: 10.3109/09537104.2010.550349.
Resveratrol (3,4',5-trihydroxystilben), a phenolic antioxidant synthesized in grapes and other plants, and also present in wine, has been suggested to help prevent cardiovascular events. In this study the influence of resveratrol on platelet aggregation during a model of hyperhomocysteinemia was investigated. We induced hyperhomocysteinemia using a reduced form of Hcys (final dose, 0.1 mM) and the most reactive form of Hcys, its cyclic thioester, homocysteine thiolactone (HTL, 1 µM). The aim of our study in vitro was also to investigate superoxide anion radical (O(2)(-)) generation after incubation of platelets with Hcys, HTL, and resveratrol. We have observed that HTL, like its precursor Hcys, stimulated the generation of (O(2)(-) in platelets and caused an augmentation of platelet aggregation induced by the strong physiological agonist thrombin. Our results in vitro also demonstrated that resveratrol reduced the toxic action of Hcys and HTL on blood platelet aggregation and superoxide anion radical production in platelets, suggesting its potential protective effects on hemostasis are negatively influenced by homocysteine and its derivatives.
白藜芦醇（3,4',5-三羟基二苯乙烯）是一种在葡萄和其他植物中合成的酚类抗氧化剂，也存在于葡萄酒中，据推测它有助于预防心血管事件。在这项研究中，研究了白藜芦醇在高同型半胱氨酸血症模型中对血小板聚集的影响。我们使用还原型 Hcys（终剂量 0.1mM）和 Hcys 最具反应性的形式，即同型半胱氨酸硫内酯（HTL，1µM）诱导高同型半胱氨酸血症。我们的体外研究还旨在研究 Hcys、HTL 和白藜芦醇孵育后血小板中超氧阴离子自由基（O(2)(-))的生成。我们观察到 HTL 与其前体 Hcys 一样，刺激血小板中（O(2)(-)的生成，并导致由强生理激动剂凝血酶诱导的血小板聚集增强。我们的体外结果还表明，白藜芦醇降低了 Hcys 和 HTL 对血小板聚集和血小板中超氧阴离子自由基生成的毒性作用，表明其对止血的潜在保护作用可能受到同型半胱氨酸及其衍生物的负面影响。
