Department of Affective and Psychotic Disorders, Medical University of Lodz, Czechoslowacka 8/10, 92-216, Lodz, Poland.
Neurochem Res. 2012 May;37(5):1057-62. doi: 10.1007/s11064-012-0707-3. Epub 2012 Jan 24.
The mechanisms of oxidative stress in schizophrenic patients are not fully understood. In the present study, we investigated the effect of elevated level of homocysteine (Hcys) on some parameters of oxidative stress, namely thiobarbituric acid reactive substances (TBARS), an index of lipid peroxidation in plasma, the level of carbonyl groups in plasma proteins, as well as the amount of 3-nitrotyrosine in plasma proteins isolated from schizophrenic patients. Patients hospitalised in I and II Psychiatric Department of Medical University in Lodz, Poland were interviewed with special questionnaire (treatment, course of diseases, dyskinesis and other EPS). According to DSM-IV criteria all patients had diagnosis of paranoid type. They were treated with antipsychotic drugs (clozapine, risperidone, olanzapine). Mean time of schizophrenia duration was about 5 years. High-performance liquid chromatography was used to analyse the total level of homocysteine in plasma. Levels of carbonyl groups and 3-nitrotyrosine residues in plasma proteins were measured by ELISA and a competition ELISA, respectively. The lipid peroxidation in plasma was measured by the level of TBARS. Our results showed that in schizophrenic patients the amount of homocysteine in plasma was higher in comparison with the control group. We also observed a statistically increased level of biomarkers of oxidative/nitrative stress such as carbonyl groups or 3-nitrotyrosine in plasma proteins from schizophrenic patients. Moreover, our experiments indicate that the correlation between the increased amount of homocysteine and the oxidative stress exists. Considering the data presented in this study, we suggest that the elevated Hcys in schizophrenic patients may stimulate the oxidative stress.
精神分裂症患者氧化应激的机制尚未完全阐明。在本研究中，我们研究了同型半胱氨酸（Hcys）水平升高对某些氧化应激参数的影响，即血浆中丙二醛（TBARS）、血浆蛋白羰基水平以及从精神分裂症患者中分离出的血浆蛋白中 3-硝基酪氨酸的量。波兰罗兹医科大学第一和第二精神病学部住院的患者接受了专门的问卷采访（治疗、疾病过程、运动障碍和其他 EPS）。根据 DSM-IV 标准，所有患者均诊断为偏执型。他们接受了抗精神病药物（氯氮平、利培酮、奥氮平）治疗。精神分裂症的平均病程约为 5 年。高效液相色谱法用于分析血浆中同型半胱氨酸的总水平。通过 ELISA 和竞争 ELISA 分别测量血浆蛋白中羰基和 3-硝基酪氨酸残基的水平。通过 TBARS 水平测量血浆中的脂质过氧化。我们的结果表明，与对照组相比，精神分裂症患者血浆中的同型半胱氨酸含量较高。我们还观察到精神分裂症患者血浆蛋白中氧化/硝化应激生物标志物如羰基或 3-硝基酪氨酸的水平呈统计学增加。此外，我们的实验表明，同型半胱氨酸增加量与氧化应激之间存在相关性。考虑到本研究中提出的数据，我们认为精神分裂症患者中升高的 Hcys 可能会刺激氧化应激。
