Department of Genetics, University of Rzeszow, Rejtana, Rzeszow, Poland.
Mech Ageing Dev. 2011 May;132(5):257-68. doi: 10.1016/j.mad.2011.04.009. Epub 2011 Apr 30.
Recently, the old horse has been proposed as a model to study telomere-dependent senescence, immunosenescence and inflamm-aging. In the present paper, we used 80 Hucul and Anglo-Arabian horses divided into 3 age groups (juvenile, adult, old) to evaluate age-dependent changes at the genomic and DNA level and in cell proliferative potential. The level of positive TUNEL cells (both apoptotic and with DNA fragmentation), oxidative DNA damage (8-oxoG immunostaining), sister chromatid exchange and bleomycin-induced chromatid breaks were significantly increased in the combined old group compared to the combined adult group. We observed a negative correlation between micronuclei formation and age, which may be associated with damaged cells undergoing apoptosis, rather than expressing micronuclei. We were unable to show any significant changes in the nuclear division index value, which reflects the proliferative status of the viable cell fraction during aging. Here, we show that breed-independent and age-associated changes in genomic stability may contribute, at least in part, to the aging process in the horse.
最近，老马被提出作为一种模型来研究端粒依赖性衰老、免疫衰老和炎症衰老。在本文中，我们使用 80 匹乌克兰马和盎格鲁-阿拉伯马，分为 3 个年龄组（幼龄、成年、老年），以评估基因组和 DNA 水平以及细胞增殖潜能的年龄依赖性变化。与成年马组合相比，衰老马组合中阳性 TUNEL 细胞（凋亡和 DNA 片段化）、氧化 DNA 损伤（8-oxoG 免疫染色）、姐妹染色单体交换和博来霉素诱导的染色单体断裂的水平显著增加。我们观察到微核形成与年龄呈负相关，这可能与受损细胞凋亡有关，而不是表达微核。我们未能显示核分裂指数值有任何显著变化，该指数反映了衰老过程中存活细胞部分的增殖状态。在这里，我们表明，与品种无关的基因组稳定性的年龄相关性变化可能至少部分导致马的衰老过程。
