KYUSHU UNIV 4609,FAC AGR,DEPT FOOD SCI & TECHNOL,FOOD SCI LAB,HIGASHI KU,FUKUOKA 812,JAPAN. KYUSHU UNIV 69,MED INST BIOREGULAT,DEPT VIROL,HIGASHI KU,FUKUOKA 812,JAPAN.
Int J Oncol. 1994 Sep;5(3):655-60.
We examined the effect of saturated fatty acids with carbon number from 2 to 10 on proliferation and survival of rat untransformed fibroblast 3Y1 cells and its transformed derivation induced by Simian virus 40 (SV-3Y1). Acetic acid (C2) had no effect on proliferation of these cells. Among medium-chain fatty acids (C6-C10), caproic acid (C6) showed the lowest proliferation inhibitory effect. Caprylic (C8), pelargonic (C9), and capric acid (C10) exerted killing activity to both 3Y1 cells and SV-3Y1 cells, and the toxicity increased with the elongation of their alkyl chains. The toxicity was a little greater to 3Y1 cells than to SV-3Y1 cells. In contrast, short-chain fatty acids caused reversible proliferation arrest in 3Y1 cells at 25 mM in propionic (C3), 5 mM in butyric (C4) and 10 mM in valeric (C5) acids. These short-chain fatty acids arrested 3Y1 cells not only in the GI phase but also in the G2 phase of the cell cycle. To SV-3Y1 cells, however, these short-chain fatty acids were cytotoxic. The cytotoxicity to SV-3Y1 cells was the greatest in butyric acid among short-chain fatty acid tested. These results suggest that short-chain fatty acids exert ploliferation-arresting activity against normal cells while exerting killing activity to the transformed cells.
我们研究了碳原子数从 2 到 10 的饱和脂肪酸对大鼠未转化成纤维细胞 3Y1 及其由猴病毒 40（SV-3Y1）诱导转化的衍生细胞增殖和存活的影响。乙酸（C2）对这些细胞的增殖没有影响。在中链脂肪酸（C6-C10）中，己酸（C6）的增殖抑制作用最低。辛酸（C8）、壬酸（C9）和癸酸（C10）对 3Y1 细胞和 SV-3Y1 细胞均具有杀伤活性，且烷基链的延长增加了其毒性。这些脂肪酸对 3Y1 细胞的毒性比 SV-3Y1 细胞略大。相比之下，短链脂肪酸在丙酸（C3）浓度为 25mM、丁酸（C4）浓度为 5mM 和缬酸（C5）浓度为 10mM 时可使 3Y1 细胞发生可逆性增殖停滞。这些短链脂肪酸不仅使 3Y1 细胞停滞在细胞周期的 G1 期，还使它们停滞在 G2 期。然而，这些短链脂肪酸对 SV-3Y1 细胞具有细胞毒性。在所测试的短链脂肪酸中，丁酸对 SV-3Y1 细胞的细胞毒性最大。这些结果表明，短链脂肪酸对正常细胞具有增殖抑制活性，而对转化细胞具有杀伤活性。
