Yamada K, Mitsui T, Okuda A, Kimura G, Sugano M
KYUSHU UNIV 69,MED INST BIOREGULAT,DEPT VIROL,HIGASHI KU,FUKUOKA 812,JAPAN.
Int J Oncol. 1993 Jan;2(1):89-93. doi: 10.3892/ijo.2.1.89.
Toxicity of polyphenols against rat 3Y1 fibroblasts and the cells transformed by human adenovirus (Ad12-3Y1), its EIA gene (EIA-3Y1), or simian virus 40 (SV-3Y1) was examined. Among the diphenol compounds examined, pyrocatechol (o-diphenol) and hydroquinone (p-diphenol) showed selective toxicity against Ad12-3Y1 and EIA-3Y1 cells, while resorcinol (m-diphenol) showed a much weaker non-specific toxicity against these cells. Another o-diphenol (dopamine) and triphenols (gallic acid and pyrogallol) were less toxic but showed selective toxicity. At lower concentrations where they were not toxic, all polyphenols attenuated toxicity of phosphatidylcholine against EIA-3Y1 cells. Among antioxidants examined, ascorbic acid reduced the toxicity of pyrocatechol, but alpha-tocopherol and butyrated hydroxytoluene did not. Oxidation of pyrocatechol was not enhanced in the presence of 3Y1 or EIA-3Y1 cells and their homogenates. These results suggest that the selective toxicity of polyphenols against Ad12-3Y1 and E1A-3Y1 cells is not related to their oxidation velocity but other factors such as the activity of active oxygen-scavenging enzymes.
检测了多酚类物质对大鼠3Y1成纤维细胞以及由人腺病毒（Ad12 - 3Y1）、其EIA基因（EIA - 3Y1）或猿猴病毒40（SV - 3Y1）转化的细胞的毒性。在所检测的二酚化合物中，邻苯二酚（邻二酚）和对苯二酚（对二酚）对Ad12 - 3Y1和EIA - 3Y1细胞表现出选择性毒性，而间苯二酚（间二酚）对这些细胞表现出弱得多的非特异性毒性。另一种邻二酚（多巴胺）和三酚（没食子酸和焦性没食子酸）毒性较小但表现出选择性毒性。在它们无毒的较低浓度下，所有多酚类物质都减弱了磷脂酰胆碱对EIA - 3Y1细胞的毒性。在所检测的抗氧化剂中，抗坏血酸降低了邻苯二酚的毒性，但α - 生育酚和丁基化羟基甲苯没有。在3Y1或EIA - 3Y1细胞及其匀浆存在的情况下，邻苯二酚的氧化没有增强。这些结果表明，多酚类物质对Ad12 - 3Y1和E1A - 3Y1细胞的选择性毒性与其氧化速度无关，而是与其他因素如活性氧清除酶的活性有关。
