Klinkhamer J M, Mitchell M D
J Dent Res. 1979 Jan;58(1):531-4. doi: 10.1177/00220345790580011501.
Benzidine dihydrochloride in the presence of dimethylsulfoxide reacts with myeloperoxidase contained within the granules of polymorphonuclear leukocytes obtained from the oral cavity. A blue meriquinoid oxidation product is formed in the presence of hydrogen peroxide, which can be extracted from the granules with a combination of methyl alcohol and dimethylsulfoxide. A stable yellow-brown pigment is formed which can be measured spectrophotometrically. The values are expressed in optical density units and correlate with the Orogranulocytic Migratory Rate units obtained by other means. Consequently, the spectrophotometric method is an additional non-subjective technique available for the evaluation, monitoring, and indexing the inflammatory periodontal disease.
在二甲基亚砜存在的情况下，盐酸联苯胺与从口腔获取的多形核白细胞颗粒中所含的髓过氧化物酶发生反应。在过氧化氢存在的情况下会形成蓝色醌型氧化产物，该产物可用甲醇和二甲基亚砜的混合物从颗粒中提取出来。会形成一种稳定的黄褐色色素，可用分光光度法进行测定。这些值以光密度单位表示，并与通过其他方法获得的嗜中性粒细胞迁移率单位相关。因此，分光光度法是一种可用于评估、监测和索引炎症性牙周病的额外的非主观技术。
