David F, Levy R, Lucotte G
Laboratoire d'Analyses Médicales du Dr Claude Lévy, Paris, France.
Mol Cell Probes. 1990 Feb;4(1):53-61. doi: 10.1016/0890-8508(90)90039-3.
Cervical biopsies collected from 105 consecutive patients participating in a prospective follow-up study for cervical human papillomavirus (HPV) infections were tested for the presence of HPV 6, 11, 16 and 18 DNA by the slot-blot hybridization method. Detectable HPV types 6 and 11 could be distinguished in high stringency hybridization assay. The overall HPV-DNA detection rate was 35.2% in our group of patients. The slot-blot technique is more rapid than Southern-blotting procedure and well suited for analysis of multiple specimens.
通过斑点杂交法，对连续参与一项关于宫颈人乳头瘤病毒（HPV）感染的前瞻性随访研究的105例患者采集的宫颈活检组织进行HPV 6、11、16和18型DNA检测。在高严格度杂交试验中可区分出可检测到的HPV 6型和11型。我们的患者组中HPV-DNA总体检出率为35.2%。斑点杂交技术比Southern印迹法更快，非常适合于多个样本的分析。
