Melki R, Khoury B, Catalan F
Department of Microbiology and Immunology, Institut Alfred Fournier, Paris, France.
J Med Virol. 1988 Oct;26(2):137-43. doi: 10.1002/jmv.1890260205.
We examined 161 human tissue samples using the spot hybridization technique with nonradioactive labeled DNA probes of human papillomavirus (HPV). Whole cells were spotted on nitrocellulose filters; DNA of the cells was denatured and fixed to the filter. Then the DNA spots were hybridized to nonradioactive labeled DNA and monitored by a sandwich immunoenzymatic reaction. This technique is simple, sensitive, specific, requires no special equipment, and can be used in clinical settings. HPV DNA was found in 92% of samples in which, on the basis of histologic and colposcopic criteria, its presence was suspected, as well as in 31 samples where it was not suspected.
我们使用人乳头瘤病毒（HPV）非放射性标记DNA探针的斑点杂交技术检测了161份人体组织样本。将全细胞点样于硝酸纤维素滤膜上；细胞DNA经变性后固定于滤膜上。然后将DNA斑点与非放射性标记DNA杂交，并通过夹心免疫酶反应进行监测。该技术简单、灵敏、特异，无需特殊设备，可用于临床。在92%根据组织学和阴道镜标准怀疑存在HPV的样本中发现了HPV DNA，在31份未怀疑存在HPV的样本中也发现了该病毒。
