Addison C, Jenkins J R, Stürzbecher H W
Cell Proliferation Laboratory, Marie Curie Cancer Research Institute, Oxted, Surrey, UK.
Oncogene. 1990 Mar;5(3):423-6.
We have identified a region of human p53 protein with striking homology to a sequence motif on Simian Virus 40 T antigen which includes the nuclear localisation signal. Mutation of basic amino acid residues in this region of p53 (residues 312 to 323; SSSPQPKKKP) compromises transport of p53 protein to the nucleus. The sequence functions efficiently as a nuclear localisation signal when fused to E. coli beta galactosidase. Serine 315 within this p53 structural motif is phosphorylated in vitro by the cell cycle kinase p34cdc2. Thus in both T antigen and p53, nuclear localisation signal and p34cdc2 kinase acceptor residue map to a contiguous region of primary amino acid sequence.
我们已经在人类p53蛋白中鉴定出一个区域，该区域与猿猴病毒40 T抗原上的一个序列基序具有显著的同源性，该基序包括核定位信号。p53这个区域（第312至323位氨基酸残基；SSSPQPKKKP）中的碱性氨基酸残基发生突变会损害p53蛋白向细胞核的转运。当与大肠杆菌β-半乳糖苷酶融合时，该序列作为核定位信号有效地发挥作用。在这个p53结构基序中的丝氨酸315在体外被细胞周期激酶p34cdc2磷酸化。因此，在T抗原和p53中，核定位信号和p34cdc2激酶受体残基都位于一级氨基酸序列的相邻区域。
