Milner J, Cook A, Mason J
Department of Pathology, University of Cambridge, UK.
EMBO J. 1990 Sep;9(9):2885-9. doi: 10.1002/j.1460-2075.1990.tb07478.x.
The normal functioning of p53 is thought to involve p53 target proteins. We have previously identified a cellular 35 kd protein associated with p53 and now report evidence identifying this 35 kd protein as p34cdc2, product of the cell cycle control cdc2 gene. The association between p53 and p34cdc2 was detected in SV3T3 and T3T3 cell lines, both expressing the wild-type p53 phenotype, and in 3T3tx cells, expressing 'mutant' p53 phenotype. Binding of the mutant p53 phenotype with p34cdc2 was greatly reduced relative to wild-type. Complexes of p53-p34cdc2 may represent inactivation or activation of either component. The p34cdc2 kinase functions at cell cycle control points and is necessary for entry and passage through mitosis. It also operates in G1 and is involved in the commitment of cells into the proliferative cycle. Since we were unable to detect p53-p34cdc2 complexes in mitotic cells we propose that the interaction between p53 and p34cdc2 may be functional in cell growth control, possibly to promote or to suppress cell proliferation.
p53的正常功能被认为涉及p53靶蛋白。我们之前鉴定出一种与p53相关的细胞35kd蛋白，现在报告证据表明这种35kd蛋白是p34cdc2，即细胞周期调控cdc2基因的产物。在表达野生型p53表型的SV3T3和T3T3细胞系以及表达“突变型”p53表型的3T3tx细胞中均检测到p53与p34cdc2之间的关联。相对于野生型，突变型p53表型与p34cdc2的结合大大减少。p53-p34cdc2复合物可能代表其中任何一种成分的失活或激活。p34cdc2激酶在细胞周期控制点发挥作用，是进入和通过有丝分裂所必需的。它也在G1期起作用，并参与细胞进入增殖周期的过程。由于我们无法在有丝分裂细胞中检测到p53-p34cdc2复合物，我们提出p53与p34cdc2之间的相互作用可能在细胞生长控制中起作用，可能是促进或抑制细胞增殖。
