Department of Endodontics, Nihon University School of Dentistry at Matsudo, 870-1, Sakaecho, Nishi-2, Matsudo, Chiba 271-8587, Japan.
J Clin Biochem Nutr. 2011 May;48(3):209-13. doi: 10.3164/jcbn.10-85. Epub 2011 Apr 26.
The purpose of this study was to investigate the effects of antioxidant biofactor (AOB) on reactive oxygen species (ROS). Generation of superoxide radical (O(2) (•-)) and hydroxyl radical ((•)OH) was determined using an electron spin resonance (ESR) spin-trapping method. AOB was added at different concentrations to these free radical generating systems. The generation of both O(2) (•-) and (•)OH was scavenged by the addition of AOB in a dose-dependent manner. These results indicate that AOB has strong antioxidant properties against these radicals. We further investigated the anti-oxidative effect of AOB on human gingival fibroblasts (HGFs). HGFs were treated for 3 h with α-MEM containing a combination of AOB and H(2)O(2) (AOB + H(2)O(2) group), containing H(2)O(2) (H(2)O(2) group), or containing AOB alone (AOB group). Non-stimulated HGFs were used as a control group. The number of surviving cells was in the order of the AOB group > control group > AOB + H(2)O(2) group > H(2)O(2) group. The level of expression of type I collagen mRNA and production of collagen were also in the order of the AOB group > control group > AOB + H(2)O(2) group > H(2)O(2) group. In conclusion, our results suggest that AOB may protect HGFs against oxidative stress by reducing stress-induced ROS.
本研究旨在探讨抗氧化生物因子（AOB）对活性氧（ROS）的影响。采用电子自旋共振（ESR）自旋捕获法测定超氧自由基（O2（•-））和羟自由基（•OH）的生成。将 AOB 以不同浓度加入这些自由基生成系统。O2（•-）和（•OH）的生成均随 AOB 的加入呈剂量依赖性清除。这些结果表明 AOB 对这些自由基具有很强的抗氧化特性。我们进一步研究了 AOB 对人牙龈成纤维细胞（HGFs）的抗氧化作用。HGFs 用含 AOB 和 H2O2（AOB+H2O2 组）、含 H2O2（H2O2 组）或仅含 AOB（AOB 组）的 α-MEM 处理 3 h。未刺激的 HGFs 用作对照组。存活细胞数的顺序为 AOB 组＞对照组＞AOB+H2O2 组＞H2O2 组。I 型胶原 mRNA 的表达水平和胶原的产生也呈现 AOB 组＞对照组＞AOB+H2O2 组＞H2O2 组的顺序。总之，我们的结果表明，AOB 可能通过减少应激诱导的 ROS 来保护 HGFs 免受氧化应激。
