Mechanical and Aerospace Engineering Department, University of California, Los Angeles, CA 90095-1597, USA.
Lab Chip. 2011 Jul 7;11(13):2247-54. doi: 10.1039/c1lc20197b. Epub 2011 May 12.
We report a novel microfluidic chamber incorporating fluid ports with active suction to achieve localized chemical stimulation of brain slices. A two-level soft-lithography process is used to fabricate fluid ports with integrated injection and suction holes that are connected to underlying microchannels. Fluorescence imaging, particle tracking velocimetry, and cell staining are used to characterize flows around a fluid port with or without active suction to validate effective localization of injected chemicals. To demonstrate biological applicability of the chamber, we show an induction of cortical spreading depression (CSD) waves in mouse brain slices through controlled focal delivery of potassium chloride solution.
我们报告了一种新颖的微流控室，该微流控室采用带有主动抽吸的流体端口，以实现脑片的局部化学刺激。采用两级软光刻工艺来制造带有集成注射和抽吸孔的流体端口，这些孔与下面的微通道相连。荧光成像、粒子跟踪测速法和细胞染色用于对带有或不带有主动抽吸的流体端口周围的流动进行特征描述，以验证注入化学物质的有效定位。为了展示该腔室的生物学适用性，我们通过控制氯化钾溶液的焦点递送，在小鼠脑片中诱导了皮质扩散性抑制（CSD）波。
